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HTRF Human CXCL10 Detection Kit, 10,000 Assay Points
HTRF Human CXCL10 Detection Kit, 10,000 Assay Points
HTRF Human CXCL10 (IP-10) Detection Kit
The HTRF human CXCL10 (IP-10) kit is designed for the quantification of human CXCL10 release in cell supernatant.
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
| Sample Volume | 16 µL |
The HTRF human CXCL10 (IP-10) kit is designed for the quantification of human CXCL10 release in cell supernatant.
Product Variants
Unit Size: 500 Assay Points
Part #:
62HCX10PEG
List Price
USD 1,149.33
Unit Size: 10,000 Assay Points
Part #:
62HCX10PEH
List Price
USD 10,722.18
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Human CXCL10 Detection Kit, 10,000 Assay Points
HTRF Human CXCL10 (IP-10) Detection Kit
HTRF Human CXCL10 Detection Kit, 10,000 Assay Points
Product information
Overview
Also called IP-10 (interferon gamma-induced protein 10) or small-inducible cytokine B10, CXCL10 is a chemokine secreted by monocytes, fibroblasts, and endolial cells in response to IFN gamma. IP-10's main effect is to promote cell migration through Calcium signaling. Target cells include macrophages, T cells, NK cells, and dendritic cells. CXCL10 has significant anti-tumor effects, as well as anti-angiogenic effects.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
16 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Cytokines
|
| Target Species |
Human
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Oncology & Inflammation
|
| Unit Size |
10,000 Assay Points
|
Video gallery
HTRF Human CXCL10 Detection Kit, 10,000 Assay Points
HTRF Human CXCL10 Detection Kit, 10,000 Assay Points
Citations
How it works
Assay principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
Revvity also worked with Myassays.com to help you in your data analysis.
Assay details
Technical specifications of human CXCL10 kit
| Sample size | 16 µL |
|---|---|
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
| LOD &LOQ (in Diluent) | 7 pg/mL & 26 pg/mL |
| Range | 26 – 5,000 pg/mL |
| Time to result | 2h at RT |
| Species | Human only |
Analytical performance
Intra and inter assay
Intra-assay (n=24)
| Sample | Mean [CXCL10] (pg/mL) | CV |
|---|---|---|
| 1 | 93 | 9% |
| 2 | 2383 | 3% |
| 3 | 4376 | 3% |
| Mean CV | 5% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
| Sample | [CXCL10] (pg/mL) | Mean (delta R) | CV |
|---|---|---|---|
| 1 | 97 | 303 | 7% |
| 2 | 470 | 1255 | 8% |
| 3 | 2273 | 4054 | 11% |
| Mean CV | 9% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
Resources
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Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
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