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PhenoVue 633 Lysosomal stain
PhenoVue 633 Lysosomal stain
PhenoVue™ 633 lysosomal stain is a fluorescent dye which accumulates in acidic vesicles, such as lysosomes. The bright deep red fluorescence associated with PhenoVue 633 lysosomal stain are validated for use in imaging microscopy and high-content screening systems.
Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue 633 lysosomal stain, has a maximum excitation wavelength of 633 nm and a maximum emission wavelength of 655 nm.
View our extensive validation data in the Product Information Sheet within the Resources tab below.
| Feature | Specification |
|---|---|
| Color | Red |
| Filter | Cy5 |
| Organelle and Cell Compartment | Lysosomes |
PhenoVue™ 633 lysosomal stain is a fluorescent dye which accumulates in acidic vesicles, such as lysosomes. The bright deep red fluorescence associated with PhenoVue 633 lysosomal stain are validated for use in imaging microscopy and high-content screening systems.
Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue 633 lysosomal stain, has a maximum excitation wavelength of 633 nm and a maximum emission wavelength of 655 nm.
View our extensive validation data in the Product Information Sheet within the Resources tab below.
PhenoVue 633 Lysosomal stain
PhenoVue 633 Lysosomal stain
Product information
Overview
Lysosomes are abundant organelles which contain more than sixty acidic enzymes. Lysosomes play essential role in macromolecules and organelles break down through the autophagy-lysosomal system. Defective regulation of lysosomal function has been reported in several diseases, such as lysosomal storage disorders and neurodegenerative diseases, including Alzheimer’s and Parkinson’s. PhenoVue 633 lysosomal stain is a pH-sensitive probe which accumulates in acidic vesicles such as lysosomes, resulting in bright and specific lysosomal fluorescent staining. PhenoVue 633 lysosomal stain can be used to visualize lysosomes in immunofluorescence, as well as high-content analysis and screening applications.
Additional product information
Features
| Numbers of vials per unit | 10 |
|---|---|
| Quantity or volume per unit | 5.5 µg |
| Form | Desiccated |
| Storage | -16 °C |
| Recommended working concentration | 0.055 µg/mL |
| Maximum excitation wavelength | 633 nm |
| Maximum emission wavelength | 655 nm |
| Common filter set | Cy5 |
| Live cell staining | Yes |
| Fixed cell staining | No |
| Equivalent number of microplates | 3-10 x 96-well microplates 3-10 x 384-well microplates 5-16 x 1536-well microplates |
Specifications
| Color |
Red
|
|---|---|
| Form |
Desiccated
|
| Maximum Emission Wavelength (Emmax) |
655 nm
|
| Maximum Excitation Wavelength (Exmax) |
633 nm
|
| Application |
High Content Imaging
Microscopy
|
|---|---|
| Brand |
PhenoVue™
|
| Detection Modality |
Fluorescence
|
| Filter |
Cy5
|
| Organelle and Cell Compartment |
Lysosomes
|
| Quantity |
10 x 5.5 µg
|
| Sample Type |
Live samples only
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Storage Conditions |
-16 °C or below, protected from light
|
| Type |
Individual Reagent
|
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Resources
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This flyer describes Revvity's PhenoVue cellular imaging reagents.
In living cells, PhenoVue lysosomal stains accumulate in acidic vesicles such as lysosomes, resulting in bright and specific...
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