Automated Methods: RNA Library Preparation Kits

RNA-Seq for transcriptome studies, a game-changer in gene expression analysis

By measuring the expression of thousands of genes in a single assay, RNA-Seq is an efficient tool to discover the complexity of biological pathways.

The preparation of RNA samples for sequencing however requires multiple manipulations to isolate and fragment mRNA, convert the RNA to double-stranded cDNA, and efficiently ligate the appropriate index adapters to produce paired-end libraries. Additionally, obtaining high-quality data from degraded RNA or FFPE-derived RNA samples can be challenging (Formalin-Fixed Paraffin-Embedded samples).

Automating your RNA library prep has distinct advantages over the manual workflow, including avoidance of sample tracking errors and reduction of sample-to-sample variability while dramatically increasing throughput.

Revvity provides a complete portfolio of NGS library preparation kits and barcodes designed to increase the flexibility and speed of library prep for customers utilizing Illumina® and Element Biosciences® sequencing platforms.

We continuously add new qualified methods, so please contact us if you do not see the one you need.

For research use only. Not for use in diagnostic procedures.

RNA Library Preparation Kits automated on Revvity liquid handlers

Vendor	Automated qualified methods	Sciclone G3 NGSx	Sciclone G3 NGSx iQ	Zephyr G3 NGS	Fontus NGS 96
10X Genomics®	Chromium® Single Cell 3' Library Kit v3			X
Agilent®	SureSelect® Strand-Specific RNA Library Prep Kit		X
Agilent®	SureSelect® XT HS2 RNA		In Dev
Illumina®/ Epicentre	Ribozero™/Scriptseq™ Stranded RNA Kit	X
Illumina®	RNA Prep with Enrichment (L) Tagmentation	X	X
Illumina®	Stranded mRNA Prep, Ligation Kit	X	X
Illumina®	Stranded Total RNA Prep, Ligation with Ribo-Zero Plus	X	X*
Illumina®	TruSeq® RNA Access Kit	X
Illumina®	TruSeq® RNA Library Prep Kit v2	X	X	X
Illumina®	TruSeq® Stranded mRNA (poly-A selection) Kit	X	X	X
Illumina®	TruSeq® Stranded Total RNA™ Kit	X	X	X
Illumina®	TruSeq® RNA Library Prep Kit v2	X	X	X
Illumina®	TruSight Oncology 500	X*
Illumina®	TruSight Oncology 500 HT				In Dev
Lexogen®	autoSENSE™ mRNA-Seq Library Prep Kit for Illumina® Sequencing	X
New England Biolabs® / NEB®	NEBNext® Enzymatic Methyl-seq Library Prep	X	X
NEB®	NEBNext® Ultra™ RNA Library Prep Kit for Illumina® Sequencing	X		X
NEB®	NEBNext® Ultra™ RNA II Library Prep Kit for Illumina® Sequencing	X
NuGen® / Tecan®	Ovation® Human Blood RNA-Seq Library Systems	X
NuGen® / Tecan®	Ovation® Human FFPE RNA-Seq Multiplex System	X
NuGen® / Tecan®	Ovation® RNA-Seq System v2	X
NuGen® / Tecan®	Ovation® Universal RNA-Seq System (strand-specific)	X
Oxford Nanopore Technologies (ONT)	PCR Tiling of SARS-CoV-2 Virus with rapid barcoding and Midnight RT PCR Expansion	X
PacBio®	HiFiViral SARS-CoV-2	[X]
QIAGEN®	QIAseq® DIRECT SARS-CoV-2	X	X
QIAGEN®	QIAseq® miRNA	X	X*
Revvity	NEXTFLEX™ Rapid Directional RNA-Seq Kit with Poly(A) Beads 2.0	X	X	X
Revvity	NEXTFLEX™ Rapid Directional RNA-Seq Kit 2.0 with RiboNaut™ rRNA Depletion Kit	X	X	X
Roche®	KAPA mRNA HyperPrep™ Kit	X		X
Roche®	KAPA RNA HyperPrep™ Kit	X	X	X
Roche®	KAPA RNA HyperPrep ™ Kit with RiboErase™ (HMR)	X		X
Roche®	Stranded mRNA-Seq Kit	X		X
Roche®	Stranded RNA-Seq Kit	X		X
Roche®	Stranded RNA-Seq Kit with RiboErase™	X		X
ScaleBio	ScaleBio Single Cell RNA Sequencing Kit v1.1				Coming
Takara® formerly Clontech	SMART-Seq® HT Kit		NGSx HT
Takara®	SMART-Seq® HT PLUS Kit			X
Takara®	SMART-Seq® v4 PLUS Kit	In Dev
Takara®	SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing	X
Takara®	SMARTer® Stranded Total RNA-Seq Kit v2 Pico Input Mammalian		X
Takara®	SMARTer® Stranded Total RNA-Seq Kit v3 Pico Input Mammalian			X
Twist®	cDNA Library Preparation Kit for ssRNA Virus Detection	X	X
Watchmaker Genomics	Watchmaker mRNA Library Prep Kit	[X]
Watchmaker Genomics	Watchmaker RNA Library Prep Kit with Polaris Depletion	In Dev