AAV Vector Manufacturing for Gene Therapy Development and Research Applications
Adeno-associated viruses (AAV) are highly versatile tools for gene therapy development and other research applications. They are used widely for their ability to deliver genetic material to a variety of tissues with high specificity and efficiency.
Revvity’s gene delivery key advantages:
- Expert Consultancy: Personalized planning and consultation to help meet the unique needs of each project.
- Quality and Consistency: Standards for reliable batch-to-batch quality.
- Customizable QC Packages: Tailored to work with the specific requirements of your research or clinical application.
- Scalability: From research-grade to GMP-ready production scales.
For research use only. Not for use in diagnostic procedures.
Recombinant AAV vectors
AAV Design Recommendations:
- AAV vectors are available in various serotypes, each with unique tissue tropism. Our experts can guide you in selecting the optimal serotype for targeting specific tissues (e.g., AAV6 for cardiac tissue).
- Selection of tissue-specific promoters is crucial for targeting gene expression to specific cell types within a tissue. We offer a variety of promoters validated for in vivo use, allowing for precise control over gene expression.
AAV services
Revvity offers a comprehensive suite of services for AAV vector generation, including:
Upstream Processes:
Utilizing adherent or suspension HEK293 cells for vector production with either two-plasmid or three-plasmid packaging systems.
Downstream Processes:
Flexible purification options:
- Step Purification: Iodixanol gradient ultracentrifugation.
- Step Purification: Affinity chromatography followed by iodixanol gradient ultracentrifugation for higher purity, ideal for late-stage preclinical applications.
Quality Control Overview
Our Quality Control (QC) packages include various assays to assess the integrity and purity of AAV vectors, such as genome titration (qPCR/ddPCR), capsid titration (ELISA), and purity checks (SDS PAGE). Additional assays, such as sterility and mycoplasma tests, are available upon request.
