Adenovirus
Adenoviral vectors
Adenoviral vectors offer high levels of transgene expression in broad cell types, including dividing and non-dividing cells. Adenoviral vectors are the method of choice whenever fast and transient gene expression for a very large gene or several genes is required, since the expression cassette has a total capacity of 7.5 kb. This provides space for flexible vector design with multiple transgenes delivered in one vector.
Vaccine development is another popular application for adenoviral vectors. The ability of adenovirus to induce immune response in vivo makes it a great tool for vaccine studies.
Revvity’s gene delivery portfolio, where SIRION Biotech’s expertise lives on, offers three technology platforms for supporting gene therapy developers working with adenovirus vectors.
Revvity has licensed its Adenovirus Technology to Danish Startup, HERVOLUTION (formerly InProTher ApS) for its Novel Immunotherapy Design Targeting Endogenous Retrovirus (ERV). Learn more
General adenovirus characteristics:
- Transient gene expression in vivo and in vitro
- No host genome integration
- Large packaging capacity up to 7.5 kb gives a space for multiple gene expression cassettes
- Reliable gene delivery into dividing and non-dividing cells with almost 100% efficiency
- Fast results- gene expression
- Adenovirus remains epichromosomal and therefore does not integrate in the genome nor interfere with other host genes
For research use only. Not for use in diagnostic procedures.
Ad19a/64 vector
Key benefits of our proprietary Ad19a/64 vector (Subtype D) include:
- High gene transfer in human Dendritic cells and muscle cells due to its unique receptor affinities
- Leverage its low seroprevalence within the human population
- Advancements such as improved CD4 immune responses over Ad5 vectors
- Overcome limitations due to CAR dependent transduction
BAC technology – building viral vectors from scratch
Classical cloning strategies are difficult and largely ineffective for generating recombinant viral vectors. Our proprietary adenoviral bacterial artificial chromosome (BAC) viral cloning technology offers a refined solution for generation of recombinant adenoviral genomes and expression libraries benefiting from the single copy origin of replication of BAC vectors helping to stabilize the large adenoviral genome.
- Generation of large AV expression libraries.
- Cloning of your gene of interest (GOI) into the pO6A5 shuttle vector
- FLP mediated recombination in E.coli BA5 FRT cells to obtain your desired Ad5 E1/E3 deleted serotype
- PAC I digestion of purified recombinant BAC DNA
- Virus reconstitution in HEK 293 cells
AdenoBOOST™ transduction enhancer
AdenoBOOST technology is a peptide-based solution which is added to the adenovirus particles prior to transduction. It bridges the adenovirus surface to the cell membrane, increasing transduction efficiency. AdenoBOOST technology is ideal to enhance gene expression in cell types that lack the primary adenovirus CAR-receptor, and can be used for preclinical research.
Benefits of the AdenoBOOST technology:
- Enhanced expression
- Applicable to most cell types without species limitations
- Reduces the necessary amount of adenovirus particles for any application
Adenovirus manufacturing
Revvity offers several production stages for preclinical applications – from cloning, vector development and manufacture to simple amplification of existing vector stock. We can adapt the production parameters to help you meet the needs of your project.
Please contact us if you’d like to speak to our specialists and learn more about any of our Adenovirus Technologies.
