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HTRF Human MANF Detection Kit, 500 Assay Points

The human MANF kit is designed for the rapid quantification of Mesencephalic Astrocyte-derived Neutrophic Factor in cell supernatants and serum.

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Feature	Specification
Application	Protein Quantification
Sample Volume	10 µL

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The human MANF kit is designed for the rapid quantification of Mesencephalic Astrocyte-derived Neutrophic Factor in cell supernatants and serum.

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Product Variants

Part #:

63ADK056PEG

List Price

USD 977.40

Part #:

63ADK056PEH

List Price

USD 12,243.50

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product information

Overview

Mesencephalic astrocyte-derived neurotrophic factor (MANF) (also recognized as ARMET or arginine-rich mutated in early stages of tumors) belongs to the fourth family of neurotrophic factors. MANF is an endoplasmic reticulum (ER) resident protein highly expressed in the developing cortex that plays an important role in protecting and repairing dopaminergic neurons in the rat 6-hydroxydopamine Parkinson disease model. MANF is also involved in protecting against various forms of endoplasmic reticulum stress. The human MANF kit enables the fast detection of MANF in cell supernatants and serum.

Specifications

Other Specifications

Application	Protein Quantification
Brand	HTRF
Detection Modality	HTRF
Product Group	Kit
Sample Volume	10 µL
Shipping Conditions	Shipped in Dry Ice
Target Class	Biomarkers
Target Species	Human
Technology	TR-FRET
Therapeutic Area	Neuroscience
Unit Size	500 Assay Points

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Citations

View product citations for 63ADK056PEG on CiteAb

How it works

Assay principle

Human MANF is measured using a sandwich immunoassay involving two specific human MANF antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of MANF present in the sample.

Assay protocol

The MANF assay features a streamlined protocol with only one incubation step following the sample/calibrator and Antibody conjugates dispensing. The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. This protocol requires a single 4-hour incubation period at RT.