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Omni Bead Ruptor bead mill homogenizer protocols

Omni Bead Ruptor bead mill homogenizer protocols

Various tissues and sample types were homogenized and assessed for analytes using the portfolio of Omni Bead Ruptor bead mill homogenizers. Below is our list of resulting performance-verified protocol snapshots. Note that all listed protocols are only recommended starting points from our or external lab experiences, and labs will need to verify their own protocols for their own workflows.

For research use only. Not for use in diagnostic procedures.

Sample Types

Human (Homo sapien) tissue

Matrix Amount Analyte Bead media Vessel Protocol Omni unit Consumable or accessory Cat #
Blood 0.8 mL Protein, DNA 0.1 mm ceramic 2 mL tube, screw cap

1. 30 Hz for 2.5 mins, 2 cycles

Note: Flip tube holder 180 ° after each cycle

 Bead Ruptor 96 19-632
Teeth One tooth DNA 2 x 10 mm stainless steel milling ball 25 mL milling jar

1. 20 Hz, 1 min

Note: Place jar in freezer for 10 min prior to use

 Bead Ruptor 96  27-203; 27-004

Animal tissue

Mammals
Matrix Amount Analyte Bead media Vessel Protocol Omni unit Consumable or accessory Cat #
Bat skin (Artibeus phaeotis) 25 – 100 mg Amino acids 2.8 mm ceramic 7 mL tube 1. 6.8 m/s for 30 sec 
2. Add 3 mL of TCA to tube 
3. 6.8 m/s, 30 secs, 3 additional cycles 

Note: Trichloroacetic acid (TCA) is a protein-precipitating agent.		 Bead Ruptor Elite 19-678
Bull hair (Bos Taurus) 50 mg Steroids 2.4 mm metal 2 mL reinforced tube 1. 6.95 m/s for 50 secs, 2 cycles with 15 sec dwell Bead Ruptor Elite 19-620
Bull bone (Bos Taurus) 5 g DNA, RNA 25 mm milling ball x 1 50 mL milling jar 1. break bone (5g) into shards to fit milling jar with ball. 
2. Place jar in liquid nitrogen (3 mins) 
4. Load and process 25 Hz, 2 mins		 Bead Ruptor 96 27-006, 27-206
Cow/Bovine Articular cartilage 5 mg Protein 2.8 mm ceramic 2 mL tube 1. Add 0.5 mL Tris. 
2. 6 m/s for 30 secs, 2 cycles with 30 sec dwell		 Bead Ruptor Elite 19-628
Coyote hair (Canis latrans) 2-3 mm Hormones 2.8 mm ceramic 2mL reinforced tube 1. 6.8 m/s for 50 secs, 4 cycles Bead Ruptor Elite 19-628
Dog placenta (Canis familiaris) 1 g Small molecules 2.8 mm ceramic 7 mL tube 1. Add 2 mL of water 
2. 6 m/s for 30 sec		 Bead Ruptor Elite 19-678
Horse hair (Equus ferus caballus) 100 mg Small molecules 2.8 mm ceramic 7 mL tube 1. HIGH speed for 30 secs, 5 cycles with 30 sec dwells Bead Ruptor 12 19-678
Horse hair (Equus ferus caballus) 100 mg Small molecules 2.4 mm metal 7 mL tube 1. 6.8 m/s for 50 secs, 3 cycles with 3 min dwells Bead Ruptor Elite 19-670
Mouse lung (Mus musculus ) 200 mg Bacteria 2.8 mm ceramic 2 mL reinforced tube 1. Add 1 mL Tris. 
5.65 m/s for 30 sec, 3 cycles with 5 sec dwell		 Bead Ruptor Elite 19-628
Mouse lung, kidney, liver, heart (Mus musculus ) 25 mg RNA 2.8 mm ceramic 2 mL reinforced tube 1. Add 0.5 mL RLB buffer. 
2. 2.9 m/s for 20 secs		 Bead Ruptor 12 19-628
Mouse quadricep muscle (Mus musculus ) 50 mg mRNA 6.5 mm ceramic 2 mL reinforced tube 1. Add 0.5 mL PBS. 
2. 4.5 m/s for 30 secs, 3 cycles with 20 secs dwell on ice		 Bead Ruptor Elite 19-648, 19-682
Pig cartilage / porcine meniscus 20 mg protein 2.8 mm ceramic 2 mL reinforced tube 1. Add 0.5 mL Tris 
2. 6 m/s for 30 secs, 4 cycles with 30 secs dwell		 Bead Ruptor Elite 19-628
Pig skin (Sus domesticus) 200 mg RNA 2.8 mm ceramic 7 mL tube 1. Add 2 mL Tris. 
2. 6 m/s for 30 secs, 3 cycles with 3 min dwells on ice		 Bead Ruptor Elite 19-678
Pig skin (Sus scrofa domesticus) 10 mm2 RNA 6.5 mm ceramic (one bead) 7 mL tube 1. Add 2.5 mL ice-cold TRIzol™. 
2. 5.5 m/s for 30 secs, 2 cycles with 1 min dwell on ice		 Bead Ruptor Elite 19-651-500, 19-682
Rat fat/adipose tissue (Rattus norvegicus) 500 mg Small molecule 2.8 mm ceramic 7 mL tube 1. Add 3 mL water. 
2. 6 m/s for 30 secs		 Bead Ruptor Elite 19-678
Rat femur (bone) (Rattus norvegicus) 200 mg Small molecule 2.4 mm metal 7 mL 1. HIGH speed for 1 min, 2 cycles with 1 min dwell on ice Bead Ruptor 12 19-670
Rat brain (Rattus norvegicus) 30 mg Protein 2.8 mm ceramic 2 mL reinforced tube 1. Add 750 µL Tris 
2. Speed 4 for 45 secs		 Bead Ruptor 4  19-628
Rat brain (Rattus norvegicus) 70 mg Protein 1.4 mm ceramic 2 mL reinforced tube 1. Add 500 µL TBS 
2. 4.85 m/s for 20 secs		 Bead Ruptor Elite 19-627
Rat brain (Rattus norvegicus) 30 mg per well Protein, DNA 5 mm stainless steel (1 per well) Deep 96-well plate 1. Add 500 µL buffer 
2. Seal plate with sealing mat 
3. 20 Hz for 30 secs		 Bead Ruptor 96 27-7005, 27-520, 27-530
Rat colon (Rattus norvegicus) 3.6 g Small molecule 2.8 mm ceramic 30 mL tube 1. Add 10 mL water
2. 5 m/s for 30 secs, 3 cycles with 1.5 min dwells		 Bead Ruptor Elite 19-6358
Rat heart (Rattus norvegicus) 50 mg Protein 2.8 mm ceramic 2 mL reinforced tube 1. Add 1 mL Tris
2. HIGH for 1 min		 Bead Ruptor 12 19-628
Rat heart (Rattus norvegicus) 30 mg Protein 2.8 mm ceramic 2 mL reinforced tube 1. Add 750 µL Tris
2. Speed 4 for 45 secs, 2 cycles with 30 sec dwell		 Bead Ruptor 4 19-628
Rat heart (Rattus norvegicus) 30 mg Protein & DNA 5 mm stainless steel (1 per well) Deep 96-well plate 1. Add 500 µL preferred buffer
2. 20 Hz for 30 sec, 3 cycles		 Bead Ruptor 96 27-7005, 27-520, 27-530
Rat small intestine (Rattus norvegicus) 30 mg DNA 2.8 mm ceramic 2 mL reinforced tube 1. Add 200 µL lysis buffer
2. 5 m/s for 10 sec		 Bead Ruptor Elite 19-628
Rat small intestine (Rattus norvegicus) 30 mg Protein, DNA 5 mm stainless steel (1 per well) Deep 96-well plate 1. Add 500 µL preferred buffer
2. 20 Hz for 30 sec, 2 cycles		 Bead Ruptor 96 27-7005, 27-520, 27-530
Rat liver (Rattus norvegicus) 30 mg Protein 2.8 mm ceramic 2 mL reinforced tube 1. Add 750 µL Tris
2. Speed 4 for 45 secs		 Bead Ruptor 4 19-628
Rat liver (Rattus norvegicus) 70 mg Protein 1.4 mm ceramic 2 mL reinforced tube 1. Add 500 µL TBS
2. 5.3 m/s for 20 secs		 Bead Ruptor Elite 19-627
Rat liver (Rattus norvegicus) 5 g Small molecule 2.8 mm ceramic 30 mL tube 1. Add 15 mL water
2. 5 m/s for 45 sec, 2 cycles		 Bead Ruptor Elite 19-6358
Rat liver (Rattus norvegicus) 3 g Small molecule 2.8 mm ceramic 15 mL tube 1. Add 4 mL water
2. 5.8 m/s for 45 sec		 Bead Ruptor Elite 19-6158
Rat lung (Rattus norvegicus) 68 mg Protein 1.4 mm ceramic 2 mL reinforced tube 1. Add 500 µL TBS
2. 5.5 m/s for 20 secs		 Bead Ruptor Elite 19-627
Rat muscle (Rattus norvegicus) 55 mg Protein, DNA 2.4 mm metal 2 mL reinforced tube 1. Add 750 µL Tris
2. HIGH for 45 secs		 Bead Ruptor 12 19-620
Rat muscle (Rattus norvegicus) 30 mg Protein 2.8 mm ceramic 2 mL reinforced tube 1. Add 750 µL Tris
2. Speed 4 for 45 secs		 Bead Ruptor 4 19-628
Rat muscle (Rattus norvegicus) 50 mg Lipid 2.8 mm ceramic 2 mL reinforced tube 1. Add 1 mL water
2. 5.65 m/s for 1 min, 2 cycles		 Bead Ruptor Elite 19-628
Rat spleen (Rattus norvegicus) 60 mg Protein 1.4 mm ceramic 2 mL reinforced tube 1. Add 500 µL TBS
2. 4.85 m/s for 20 secs		 Bead Ruptor Elite 19-627
Rat trachea (Rattus norvegicus) 2 – 3 mm2 (3 – 10 mg) RNA 1.4 mm ceramic 0.5 mL tubes 1. Add 250 µL Tris
2. Speed 4 for 60 secs, 2 cycles		 Bead Ruptor 4 19-626

 

Birds
Matrix Amount Analyte Bead media Vessel Protocol Omni unit Consumable or accessory Cat #
Chicken liver (Gallus gallus domesticus) 2 g Small molecule 2.8 mm ceramic 15 mL tube 1. Add 3 mL of water 
2. 6.45 m/s for 30 secs, 3 cycles with 1 min dwells		 Bead Ruptor Elite 19-6158
Chicken liver (Gallus gallus domesticus) 5 g Small molecule 2.8 mm ceramic 30 mL tube 1. Add 10 mL of water 
2. 5 m/s for 45 secs, 2 cycles		 Bead Ruptor Elite  19-6358
Chicken liver (Gallus gallus domesticus) 200 mg Small molecule 2.8 mm ceramic 7 mL tube 1. Add 1.8 mL 50:50 Methanol:H2O 
2. 5.3 m/s for 30 secs		 Bead Ruptor Elite 19-678
Chicken egg shells (Gallus gallus domesticus) 6 g Nutritional analysis 10 mm milling ball x 4 50 mL milling jar 1. 30 Hz, 2 mins Bead Ruptor 96 27-203, 27-006
Goose feather (Anser anser domesticus) One feather (~100 mg) Steroids 2.8 mm ceramic 7 mL tube 1. Cut up feather to fit tube. 
2. 6.6 m/s for 30 secs, 5 cycles with 3 min dwells		 Bead Ruptor Elite 19-678
Goose feather (Anser anser domesticus) One feather (~100 mg) Steroids 2.8 mm ceramic 7 mL tube 1. Cut up feather to fit tube. 
2. HIGH setting for 30 secs, 5 cycles with 3 min dwells		 Bead Ruptor 12 19-678

 

Fish
Matrix Amount Analyte Bead media Vessel Protocol Omni unit Consumable or accessory Cat #
Trout head bones (Oncorhynchus mykiss) 2 g Parasite 2.8 mm ceramic 50 mL tube 1. Add 20 mL of 0.5% pepsin-HCL. 
2. 4.7 m/s for 30 secs, 3 cycles with 2 min dwells		 Bead Ruptor Elite 19-6508

 

Insects
Matrix Amount Analyte Bead media Vessel Protocol Omni unit Consumable or accessory Cat #
Beetle (Tenebrio molitor) 30 mg DNA 2.8 mm ceramic 2 mL reinforced tube 1. 350 µL preferred lysis buffer 
2. Speed 4 for 30 sec		 Bead Ruptor 4  19-628
Fruit fly (Drosophila melanogaster) (Whole) One fly (30 - 50 mg) Protein, DNA 1.4 mm ceramic 2 mL tube 1. Add 1 mL Tris 
2. 6 m/s for 30 secs		 Bead Ruptor Elite 19-627
Honey bee (Apis mellifera) (Whole) 4 bees DNA 2.8 mm ceramic 7 mL tube 1. Add 2 mL water 
2. 4 m/s for 30 secs, 2 cycles with 15 sec dwell		 Bead Ruptor Elite  19-678
Mosquito (Aedes aegypti) (Whole) ~50 mg DNA 2.4 mm stainless steel Deep 96-well plate 1. Add 1 mL CTL buffer (Omega-Biotek). 
2. 24 Hz, 5 mins. 

Note: Rotate plate halfway through cycle.		 Bead Ruptor 96 19-640, 27-520, 27-530
Wolf spider (Allocosa apora) (Whole) One spider (240 mg) Protein 1.4 mm ceramic 7 mL tube 1. Add 2 mL Tris
2. 4 m/s for 30 secs		 Bead Ruptor Elite 19-677

 

Nematodes
Matrix Amount Analyte Bead media Vessel Protocol Omni unit Consumable or accessory Cat #
Vinegar eel (Turbatrix aceti) 3 mL culture DNA 0.5 mm glass 2 mL reinforced tube 1. Spin down and resuspend in 200 µL preferred lysis buffer
2. Transfer to 2 mL tube with bead media
3. Speed 5 for 20 - 30 sec		 Bead Ruptor 4 19-622

Fungi

Matrix Amount Analyte Bead media Vessel Protocol Omni unit Consumable or accessory Cat #
Yeast (Saccharomyces cerevisiae) 3 mL culture DNA 0.1 mm ceramic 2 mL reinforced tube 1. Spin down and resuspend in 480 µL of LD buffer and lyticase solution
2. Incubate at 30 °C, add 200 µL lysis buffer + 10 µL antifoam
3. Transfer to 2 mL tube with bead mix
4. 5 m/s for 30 secs, 2 cycles with 45 sec dwell		 Bead Ruptor Elite 19-632D
Yeast (Saccharomyces cerevisiae) 40 mL culture RNA 0.1 mm ceramic 2 mL reinforced tube 1. Spin down and resuspend in 480 µL of LD buffer and lyticase solution
2. Incubate at 30 °C, add 350 µL lysis buffer + 10 µL antifoam
3. Transfer to 2 mL tube with bead mix
4. 4.2 m/s for 30 secs, 2 cycles with 45 sec dwell		 Bead Ruptor Elite 19-632D

Bacteria

Matrix Amount Analyte Bead media Vessel Protocol Omni unit Consumable or accessory Cat #
Escherichia coli 15 mL overnight culture Protein 0.1 mm ceramic 2 mL reinforced tube 1. Spin down and resuspend in 1 mL Tris
2. Transfer to 2 mL tube with bead mix
3. Speed 5 for 1 min		 Bead Ruptor 4 19-632D
Escherichia coli 3 mL overnight culture DNA 0.1 mm ceramic 2 mL reinforced tube 1. Spin down and resuspend in 200 µL Tris-EDTA and lysozyme buffer
2. Incubate at 37 °C, add 100 µL lysis buffer + 10 µL antifoam
3. Transfer to 2 mL tube with bead mix
4. 4.35 m/s for 45 secs		 Bead Ruptor Elite 19-632
Escherichia coli 1.6 mL overnight culture DNA 0.1 mm ceramic 2 mL reinforced tube 1. Spin down and resuspend in 200 µL Tris-EDTA and lysozyme buffer
2. Incubate at 37 °C, add 100 µL lysis buffer + 10 µL antifoam
3. Transfer to 2 mL tube with bead mix
4. Speed 4 for 45 secs		 Bead Ruptor 4 19-632D
Escherichia coli 6 mL overnight culture DNA, RNA 0.1 mm ceramic Deep 96-well plate 1. Spin down and resuspend in 400 µL Tris-EDTA and lysozyme buffer
2. Incubate at 37 °C
3. Split samples, 200 µL for DNA extraction and 200 µL for RNA extraction into wells of 96-well plate with bead mix
4. Add 100 µL lysis buffer + 10 µL antifoam to each well
5. 30 Hz for 2 min		 Bead Ruptor 96 27-6006