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Utilization of a mechanical homogenization-based direct-to-PCR method for influenza A virus detection

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This application note demonstrates a mechanical homogenization-based direct-to-PCR (dPCR) method for virus detection, which reduces the time and resources needed compared to traditional PCR methods. We present this alternative method using mechanical bead mill homogenization to be effective in releasing intracellular viral RNA for RT-qPCR amplification, ultimately reducing the cost and turnaround time compared to traditional extraction methods upstream of RT-PCR.

For research use only. Not for use in diagnostic procedures.

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Utilization of a mechanical homogenization-based direct-to-PCR method for influenza A virus detection

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