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AlphaLISA AlphaScreen

AlphaLISA 5 μL vs. 20 μL Sample Volume

Section
AlphaLISA 5 μL vs. 20 μL Sample Volume
Alpha EMSA Conversion
Alpha Immunogenicity
Alpha Protein-protein and Protein-nucleic Acid Interactions
Alpha Troubleshooting Tables
AlphaLISA Epigenetic Toolbox Reagents
Alpha Instrument Options and Settings
Alpha Bead Conjugation
AlphaLISA 5 μL vs. 20 μL Sample Volume
Alpha Products and Catalog Numbers
Alpha Antibody Detection and Characterization
Alpha Citations
Bead Selection and Bead Interference
The Hook Effect
Other Alpha Applications
AlphaLISA Immunoassay Kits
Preparation of Analyte-depleted Serum for Alpha Assays
Working with cell extracts and supernatants in Alpha assays
Data Analysis for AlphaLISA Immunoassays
Working with Serum and Other Biological Matrices in Alpha Assays
Create your own Alpha Assay
Determining Kd With an Alpha Assay
AlphaLISA and AlphaScreen No-wash Assays
Buffer Selection for Alpha Assays

Overview

  • Increasing the volume of the sample while keeping the overall reaction volume can increase the sensitivity of the assay. This is because you are adding more molecules of analyte into the well.
  • Please note that increasing the volume of sample in a 50 µL reaction might also increase any potential assay interference arising from your sample or matrix. We do not recommend adjusting the volume of your sample if you are using serum samples.

     

alphaLISA™- 5 μL- vs. 20 μL- Sample Volume

For research use only. Not for use in diagnostic procedures.

The information provided above is solely for informational and research purposes only. Revvity assumes no liability or responsibility for any injuries, losses, or damages resulting from the use or misuse of the provided information, and Revvity assumes no liability for any outcomes resulting from the use or misuse of any recommendations. The information is provided on an "as is" basis without warranties of any kind. Users are responsible for determining the suitability of any recommendations for the user’s particular research. Any recommendations provided by Revvity should not be considered a substitute for a user’s own professional judgment.

 

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