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MCC + QF-PCR

Test code: MCCQFP002

Test Inquiry

Test code: MCCQFP002

Test Inquiry

MCC + QF-PCR

Quantitative Fluorescent-Polymerase Chain Reaction (QF-PCR) is a laboratory technique used to amplify specific regions of DNA and quantify the amount of DNA present in those regions. Maternal Cell Contamination (MCC) - Presence of maternal cells or DNA in fetal sample/DNA is known as MCC

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Test Code	MCCQFP002
Test Summary	Quantitative Fluorescent-Polymerase Chain Reaction (QF-PCR) is a laboratory technique used to amplify specific regions of DNA and quantify the amount of DNA present in those regions. Maternal Cell Contamination (MCC) - Presence of maternal cells or DNA in fetal sample/DNA is known as MCC
Turn Around Time	7 days
Acceptable Sample Types	DNA, Isolated , Products of Conception , Whole Blood (EDTA)
NY Approved	No
Accreditations	CAP , ISO 15189 (NABL)

*TAT starts after the sample and all required sample information is received at the processing laboratory.

**The CPT codes listed are in accordance with Current Procedural Terminology, a publication of the American Medical Association, and are provided for informational purposes only. CPT coding is the sole responsibility of the billing party.

This testing service has not been cleared or approved by the U.S. Food and Drug Administration. Testing services may not be licensed in accordance with the laws in all countries. The availability of specific test offerings is dependent upon laboratory location.

Test code: MCCQFP002

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This test is not available in your region United States, please select your nearest laboratory to request more information.

Test Inquiry

Test information

Test description

Quantitative Fluorescence PCR (QF-PCR) is a molecular-based technique employed for prenatal and postnatal diagnosis of rapid aneuploidy detection(RAD) of chromosomes 13, 18, 21, X, and Y.A technique based on the amplification of chromosome-specific DNA sequences (STR, short tandem repeats) polymorphic in length, is used to determine the presence of different alleles. Employing fluorescent primers, the amplified segments can be visualized and quantified as peak areas on automated DNA scanners.QF-PCR is rapid, cost-effective, almost entirely automated, small sample size with high accuracy, and can detect most abnormalities diagnosed by conventional karyotyping. Maternal Cell Contamination (MCC)Presence of maternal cells or DNA in fetal sample/DNA is known as Maternal Cell Contamination (MCC). Highly polymorphic STR (short tandem repeat) loci are used to differentiate between fetal and maternal samples/DNA, identify extent of Maternal Cell Contamination if present and thus avoid erroneous reporting. At present, the test can confidently identify 10% Maternal Cell Contamination or greater.

Indications for testing

QFPCR

Abnormal fetal ultrasound and presenceof soft markers like hypoplastic nasalbone, Increased NT, Echogenic bowel etc.
Maternal serum screening positive forchromosome aneuploidies
NIPT positive for chromosomeaneuploidies
Parental chromosomal rearrangement ormosaicism
Previous history of recurrent miscarriages
Previous fetus/baby with chromosomalabnormality

MCC

One of the risks associated with prenatal /fetal testing is maternal cell contamination (MCC), which occurs when fetal specimen is mixed with maternal blood or tissue. The risk of MCC is associated with any fetal sample such as chorionic villus, POCs, Cord Blood, Amniotic Fluid, etc. If MCC is present, the maternal DNA may interfere with the fetal genetic testing thereby resulting in inaccurate reporting and diagnosis. This test will enable us to identify if a specific fetal sample has MCC. In order to perform this test a maternal blood sample is required along with the fetal sample.

Condition description

QFPCR

Screening result suggests aneuploidy
Advanced maternal age
One major or at least two minor fetal structural defects found on ultrasound
Previous fetus or child with aneuploidy
Parent of this pregnancy has a structural chromosome abnormality (e.g.,translocation, inversion) involving chromosome 21, 13, 18, X, or Y
Parent of this pregnancy has an extra chromosome (e.g., Down syndrome, XXX syndrome, Klinefelter syndrome)

MCC

The presence of both maternal and nonmaternal alleles for each fetal marker indicates the fetal specimen is not contaminated. MCC is confirmed when both alleles in the fetus are maternal.

Test methods and limitations

QF PCR analysis includes amplification, detection and analysis of chromosome-specific DNA sequences known as genetic markers or small tandem repeats (STRs).

MCC - Testing includes amplification of highly polymorphic short tandem repeat (STR) markers with fluorescently labelled primers; fragment sizing of the resulting PCR products using a genetic analyzer.

Detailed sample requirements

Whole Blood (EDTA)

Test Details Page
Collection Container(s)	EDTA (purple top)
Collection	Infants (< 2-years): 2 to 3 mL; Children (>2-years): 3 to 5 mL; Older children and adults: Minimum 5mL. The blood tube should be inverted several times immediately after blood collection to prevent coagulation.
Sample Condition	Store at ambient temperature. Do not refrigerate or freeze.
Shipping	Ship overnight at ambient temperature ensuring receipt within 5-days of collection.
SPECIAL SAMPLE INSTRUCTIONS	Clotted or hemolyzed samples are not accepted.

Products of Conception

Test Details Page
Collection Container(s)	Sterile, screw-top container filled with tissue culture transport medium.
Collection	All prenatal specimens will be tested for maternal cell contamination (MCC). Send maternal blood (EDTA tube) for comparison. Transport products of conception and/or fresh tissue (minimum: 10 mg) in a sterile, screw-top container filled with tissue transport medium. Do not place in formalin. If the specimen is too large for a normal collection tube, a larger sterile container can be used.
Sample Condition	If the autopsy is performed: Facia lata, diaphragm, tendon, skin, tissue from internal organs (if fresh), chest wall cartilage (particularly if macerated), or placenta from fetal side. If no autopsy is performed: Placenta from fetal side is preferred, e.g., villi. Umbilical cord is also acceptable. State clearly in the TRF whether the tissue was of fetal or placental in origin.
Shipping	Shipped immediately at ambient temperature or with a cold pack, especially under hot weather to the laboratory by overnight express with arrival Monday-Friday only. Alternatively, fresh POC and/or tissue samples can be snap-frozen and kept at -80°C. Frozen tissue samples should be shipped overnight on dry ice with arrival Monday-Friday only - NO WEEKEND SHIPMENTS!
SPECIAL SAMPLE INSTRUCTIONS	For prenatal samples, Revvity Omics will provide a shipping label to use for shipping the sample to our lab. Please call 1 (866) 354-2910 to arrange this. At this time, you will also be connected to a laboratory genetic counselor to answer any questions about the testing. For samples originating outside of the United States, please contact your nearest Revvity Omics laboratory for more information.

DNA, Isolated

Test Details Page
Collection	Required DNA Quantity by Test Type: Next Generation Sequencing (NGS):* Send >1000 ng total gDNA @ >15 ng/μL. Please ship samples in 10mM Tris. Do not use EDTA. Sanger Sequencing: Send >500 ng total gDNA @ >15 ng/μL (varies by the size of the gene and the variants requested). Non-Sanger Sequencing Tests: Send >500 ng total gDNA @ >15 ng/μL.
Sample Condition	* Required DNA Quality: High molecular weight DNA (>12kb). A260/A280 reading should be ≥ 1.8. A260/230 a ratio range of 1.8 to 2.2. Contact the laboratory for specific amounts if total ng cannot be met.
Shipping	Ship overnight at ambient temperature.
SPECIAL SAMPLE INSTRUCTIONS	Research Laboratories: DNA extracted in research laboratories is not acceptable. Only under exceptional circumstances (e.g., proband not available) will DNA extracted in a research laboratory be accepted for clinical testing. Additional testing (e.g., of other family members) may be required to confirm results. Laboratories outside the United States: Non-US laboratories are not subject to CLIA regulations and will be reviewed on a case-by-case basis. Please call to speak with a laboratory genetic counselor before submitting a DNA sample from any non-CLIA-certified laboratory. Special Notes: If extracted DNA is submitted, information regarding the method used for extraction should be sent along with the sample.

How To Order

Step 1

Choose Your Test

Select the correct test for your patient, and download and fill out the Clinical Genomics test requisition form.

Step 2

Collect Sample

Obtain a sample for testing from the patient using one of the provided Revvity Omics test packs.

Step 3

Send Samples

Send samples and all required forms back to Revvity for processing using pre-paid shipping label.

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Revvity Sites Globally

MCC + QF-PCR

MCC + QF-PCR

MCC + QF-PCR

About this test

Specifications

Test information

Test description

Indications for testing

Condition description

Test methods and limitations

Detailed sample requirements

Whole Blood (EDTA)

Products of Conception

DNA, Isolated

How To Order

Choose Your Test

Collect Sample

Send Samples

How can we help you?

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MCC + QF-PCR

MCC + QF-PCR

MCC + QF-PCR

About this test

Specifications

Revvity Sites Globally

Test information

Test description

Indications for testing

Condition description

Test methods and limitations

Detailed sample requirements

Whole Blood (EDTA)

Products of Conception

DNA, Isolated

How To Order

Choose Your Test

Collect Sample

Send Samples

1. Build Your Custom Panel

2. Complete and print the test requisition

3. Collect and Send Patient Sample

How can we help you?