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Application Note
Simultaneous detection of drug efficacy and toxicity by combining HTRF, AlphaLISA, or AlphaLISA SureFire Ultra with ATPlite
This application note demonstrates how compound’s mechanism of action and potential cytotoxic effects can be deciphered thanks to the combination of AlphaLISA™, HTRF™ or AlphaLISA™ SureFire® Ultra™ immunoassays with ATPlite™ 1step cell viability assay.
Technical Note
A simple method for preparing GPCR membrane model extracts from stable cell lines for use with the HTRF GTP Gi binding assay
This Technical Note provides a detailed method for preparing GPCR membrane model extracts from stable cell lines for use with the HTRF GTP Gi binding assay, including cell culture preparation, cell lysis, membrane extraction, and assay optimization.
Application Note
No-Wash IP1 assays are a powerful readout to characterize compounds modulating the FGFR signaling pathway in cancer drug research
This application note demonstrates that detecting the intracellular accumulation of IP1, mediated by FGFR-dependent activation of PLCγ1, can be a powerful alternative for characterizing compounds that modulate FGFR signaling in various cancer cell lines expressing FGFR1, FGFR2, and FGFR3.
Application Note
Comparison of EMT Biomarker Expression in 2D Monolayer and 3D Spheroid Cultures in a Prostate Cancer
This application note shows that AlphaLISA can be used to compare differences in protein expression levels and cellular tolerance for compound treatment between a human prostate cancer cell line grown in monolayers and those same cells grown in 3D spheroids.
Flyer
Download your ATPlite flyer
Revvity offers ATPlite™ and ATPlite 1step assays to measure cellular ATP levels, indicating cell viability and proliferation, using firefly luciferase and D-luciferin. Check out our flyer for the latest solutions in cytotoxicity, cell viability, and cell proliferation assays.
Brochure
Biologics workflow solutions
Revvity can help you discover and develop high-quality biological drug products by streamlining the biologics workflow.
Flyer
Potency detection assays and solutions
Hit key requirement guidelines with Revvity's biochemical and cell-based potency detection assays
Webinar
Streamlining sample preparation for successful single cell sequencing
Webinar about streamlining sample preparation for successful single cell sequencing
Application Note
Protocol optimization for detection and quantification of membrane-bound proteins using AlphaLISA technology
AlphaLISA kits have the capability to assess hundreds of targets, including cytokines, proteins circulating in the blood, and cellular proteins.
Technical Note
Biotin-free AlphaLISA assays for the detection of cytokines in PBMC supernatants
In this technical note, AlphaLISA biotin-free kits were used to detect and quantify the cytokines interleukin 2 (IL-2), tumor necrosis factor alpha (TNFα), interferon gamma (IFN-γ), and interleukin 6 (IL-6).
Whitepaper
Learning from our ancestors: can we be better prepared for the next pandemic?
Researchers have found evidence of an ancient viral epidemic involving host coronavirus interacting genes in East Asia more than 20,000 years ago. These findings could help countries prepare for future pandemics.
Brochure
Get Our Brochure on Luminescence Assay Technology
Get Revvity’s brochure on advanced luminescence assay technology, featuring high sensitivity and dynamic range for gene expression, cell viability, and drug development.
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