Scintillation Proximity Assay (SPA)
Combine the sensitivity of radioactive assays with the convenience of a homogeneous (no wash steps) assay using SPA technology. Scintillation proximity assay (SPA) is a homogeneous and versatile technology for the rapid and sensitive assay of a wide range of biological processes, including use with enzyme targets and receptor targets, radioimmunoassays, and molecular interactions. The assay format requires no separation steps and is amenable to automation.
SPAs using either SPA scintillation beads or SPA imaging beads can be read on scintillation counters and CCD imagers, respectively. In addition, SPA reagents are suitable for the detection of a range of isotopes, including 3H, 125I, 33P, and 14C, when used as radiolabels.
For biochemical assays such as receptor binding or enzyme activity, a scintillation proximity assay offers a more convenient, cost-effective, and safer technology for a range of applications. Furthermore, our CytoStar-T® microplates are sterile, TC-treated, clear-bottomed, white-walled microplates optimized for cell-based scintillation proximity assays.
For research use only. Not for use in diagnostic procedures.
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WGA-coated SPA beads, for capturing cell membranes in proximity-based radiometric scintillation assays.
WGA-coated SPA beads, for capturing cell membranes in proximity-based scintillation assays.
SPA (scintillation proximity assay) beads designed for the capture of [3H]AMP or [3H]GMP on specially prepared yttrium silicate beads.
SPA beads for capturing His-tagged proteins and peptides.
SPA beads for capturing His-tagged proteins and peptides.
WGA-coated SPA beads, for capturing cell membranes in proximity-based radiometric scintillation assays.
WGA-coated SPA beads, for capturing cell membranes in proximity-based radiometric scintillation assays.
Glutathione-coated SPA beads, for capturing GST-tagged proteins in proximity-based radiometric scintillation assays.
Streptavidin-coated SPA beads, for capturing biotinylated biomolecules in proximity-based radiometric scintillation assays.
SPA bead-based GTPγS binding (GPCR) assay kit.
WGA-coated SPA beads, for capturing cell membranes in proximity-based radiometric scintillation assays.
WGA-coated SPA beads, for capturing cell membranes in proximity-based scintillation assays.
![Phosphodiesterase [3H] cAMP SPA Enzyme Assay](https://resources.revvity.com/images/TRKQ7090-700x700px.jpg)
cAMP radioimmunoassay kit using Scintillation Proximity Assay (SPA) beads
Streptavidin-coated SPA beads, for capturing biotinylated molecules in proximity-based scintillation assays.
Anti-mouse-coated SPA beads, for capturing mouse IgG antibodies in proximity-based radiometric scintillation assays.
Uncoated SPA beads that interact with primary phosphate groups in nucleotides (oligos, DNA and RNA); membranes can also bind.
Glutathione-coated SPA beads, for capturing GST-tagged proteins in proximity-based scintillation assays.
Streptavidin-coated SPA beads, for capturing biotinylated biomolecules in proximity-based radiometric scintillation assays.
WGA-coated SPA beads, for capturing cell membranes in proximity-based scintillation assays.
Protein A-coated SPA beads, for capturing antibodies in proximity-based radiometric scintillation assays.
96-well clear-bottom, scintillating microplate for cell-based radiometric proximity assays.
WGA-coated SPA beads, for capturing cell membranes in proximity-based scintillation assays.
Streptavidin-coated SPA beads, for capturing biotinylated biomolecules in proximity-based radiometric scintillation assays.
Streptavidin-coated SPA beads, for capturing biotinylated biomolecules in proximity-based radiometric scintillation assays.
![Phosphodiesterase [3H] cGMP SPA Enzyme Assay](https://resources.revvity.com/images/TRKQ7100-700x700px.jpg)
cGMP radioimmunoassay kit using Scintillation Proximity Assay (SPA) beads