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PhenoVue Fluor 555 - Phalloidin

PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS. View All
PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin

PhenoVue™ Fluor 555 - Phalloidin is a fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.

PhenoVue Fluor 555 conjugated Phalloidin exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue 555 - Phalloidin has a maximum excitation wavelength of 555 nm and a maximum emission wavelength of 570 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

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Feature Specification
Color Yellow
Filter Cy3
Fluorophore PhenoVue™ Fluor 555
Organelle and Cell Compartment Actin
Request More Information

PhenoVue™ Fluor 555 - Phalloidin is a fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.

PhenoVue Fluor 555 conjugated Phalloidin exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue 555 - Phalloidin has a maximum excitation wavelength of 555 nm and a maximum emission wavelength of 570 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

Click to copy promo code to clipboard.
March lab savings - buy on Revvity.com or Revvity punchout to SAVE 10% with promo code!
MARCH10
Sale ends 3/24. Terms and conditions apply.
View product information
Product Variant
Quantity: 1 x 300 units
Part #:
CP25551
List Price
USD 545.83
Your online price:
For research use only. Not for use in diagnostic procedures.
Total list price:
USD 545.83
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PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin

Product information

Overview

Phalloidin is a toxin isolated from the fungus Amanita phalloides. It is a bicyclic peptide that specifically binds and stabilizes actin filaments.

Fluorescent Phalloidin derivatives have been extensively used for monitoring actin expression and dynamics of the cytoskeleton network. The study of actin cytoskeleton regulation is of interest in the context of several pathophysiological processes such as cancer and neurodegenerative diseases as well as fibrosis and vascular diseases.

PhenoVue Fluor 555 - Phalloidin can be used to vizualise actin filaments in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.

Quantity per pack: 300 units (10 nmoles; 13 µg)

Recommended working concentration: 165 nM (0.21 µg/ml)

Equivalent number of microplates:

  • 2 - 6 x 96-well microplates
  • 1.5 - 6 x 384-well microplates
  • 3 - 10 x 1536-well microplates

Specifications

Color
Yellow
Form
Desiccated
Maximum Emission Wavelength (Emmax)
570 nm
Maximum Excitation Wavelength (Exmax)
555 nm
Application
High Content Imaging
Microscopy
Brand
PhenoVue™
Detection Modality
Fluorescence
Filter
Cy3
Fluorophore
PhenoVue™ Fluor 555
Organelle and Cell Compartment
Actin
Quantity
1 x 300 units
Sample Type
Fixed samples only
Shipping Conditions
Shipped in Dry Ice
Storage Conditions
-16 °C or below, protected from light
Type
Individual Reagent

Image gallery

PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin

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PhenoVue Cellular Imaging Reagents Brochure
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PhenoVue Fluor - Phalloidin Conjugates Product Information Sheet

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