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Application Note
cAMP assay provides flexibility and stable pharmacology
AlphaScreen™ technology provides a quantitative detection of molecules in a homogeneous, no-wash format and can be applied to GPCR research.
Application Note
Functional GPCR Studies Using AlphaScreen cAMP Detection Kit
Read this application note to access detailed data and results on GPCR studies using AlphaScreen cAMP detection kit
Application Note
Avoiding biotin interference in AlphaLISA assays
This application note demonstrates the value of using AlphaLISA biotin-free kits to reduce the effects of biotin interference in sample and standard preparations.
Whitepaper
Alpha technologies for antibody detection and characterization
Alpha technology is homogeneous and non-radiometric with distinct features that makes it enabling in comparison to other proximity assays.
Application Note
Comparison of EMT Biomarker Expression in 2D Monolayer and 3D Spheroid Cultures in a Prostate Cancer
This application note shows that AlphaLISA can be used to compare differences in protein expression levels and cellular tolerance for compound treatment between a human prostate cancer cell line grown in monolayers and those same cells grown in 3D spheroids.
Application Note
Biochemical binding ADCC assays utilizing AlphaLISA toolbox reagents for the characterization of hIgGs and FcγR1A
In this application note we demonstrate the ease with which AlphaLISA toolbox reagents can be used to develop any FcγR binding assay across the various stages of biologics research and development, including therapeutic screening and GMP Lot Release.
Application Note
Rapid no wash assays for characterizing a mouse TIGIT/PD-L1 bispecific antibody
In this application note, we demonstrate how AlphaLISA™ assay technology can be used for bispecific antibody detection.
Application Note
A comparative study of two immunoassay platforms to determine lentivirus titer for CAR-T development
This application note demonstrates a comparative quantification of the p24 titer in a lentiviral GFP control sample using Alliance HIV-1 p24 Antigen ELISA and p24 AlphaLISA immunoassay platforms.
Application Note
Development of pharmacokinetic (PK) assays for detecting biosimilars targeting TNF alpha using AlphaLISA
In this Application Note, AlphaLISA™ assays are used for detecting biosimilars targeting TNF alpha. Learn more.
Application Note
Antigen-stimulated PBMC cytokine release measured by AlphaLISA bovine cytokine kits
This application note demonstrates the performance of AlphaLISA bovine cytokine kits by measuring the cytokine release from antigenstimulated PBMCs isolated from cow blood and shows how cytokine measurements are critical for understanding cell mediated immune responses during vaccine development.
Application Note
Simultaneous detection of drug efficacy and toxicity by combining HTRF, AlphaLISA, or AlphaLISA SureFire Ultra with ATPlite
This application note demonstrates how compound’s mechanism of action and potential cytotoxic effects can be deciphered thanks to the combination of AlphaLISA™, HTRF™ or AlphaLISA™ SureFire® Ultra™ immunoassays with ATPlite™ 1step cell viability assay.
Guide
Antibody conjugation to AlphaLISA Acceptor beads detailed protocol
Detailed protocol step-by-step: recommended reagents and materials, antibody assessment and pre-treatment, bead conjugation reaction and bead coupling optimization.
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