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Technical Note
Bioluminescence tomography - setup and sequence acquisition
This techinical note highlights how to setup up and sequence bioluminescene tomography on the IVIS Spectrum platform
Technical Note
Brief overview guide on spectral unmixing
Technical note outlining using spectral unmixing in Living Image software to isolate and quantify each individual fluorescent source for separate visualization and quantification in vivo.
Technical Note
General and technical considerations for background subtraction in 2D fluorescence using IVIS imaging systems
The technical not provides an overview on general and technical considerations for background subtraction in 2D fluorescence using IVIS imaging systems
Technical Note
Bioluminescence tomography - topography
Technical note highlighting 3D bioluminscence tomography topography on the IVIS Spectrum platform
Technical Note
Spectral unmixing using Living Image software
This technical note/guide discusses how to use the spectral unmixing tool in Living Image software
Technical Note
Kinetic analysis of bioluminescent sources for in vivo imaging
Technical highlighting kinetic analysis of bioluminescent sources for in vivo imaging
Technical Note
How to determine saturation in your in vivo optical imaging studies
This technical note outlines the prodecures for determining saturation in you in vivo optical imaging studies using the IVIS platform.
Technical Note
Working with 2D image overlay using Living Image Software
This technical note outlines loading multiple images and overlaying them, for example bioluminescent tumor with fluorescent targeted drug acquired in two separate images
Technical Note
Fluorescence tomography - Setup and sequence acquisition
This tech note will discuss how to set up an imaging experiment in Living Image for fluorescence tomography using IVIS imaging systems.
Technical Note
Transillumination fluorescence using Living Image software
This tech note will walk you through the steps of manually entering your settings for a transillumination sequence. The Living Image software include an Autoexposure setting and an Imaging Wizard and it is recommended that you use these features to simplify the setup.
Technical Note
Transillumination fluorescence - raster scan
The tech note/guide discusses raster scanning within Living Image software to faciliate faster transillumination imaging
Technical Note
Trans-illumination fluorescence - normalized transmission fluorescence
The tech note/guide discusses normalized transmission fluorescence is a technique that allows us to subtract background light leakage through thin tissue from transillumination images utilizing an extra image captured with a neutral density (ND) filter
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