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WGA PVT Special SPA Beads, 25 X 500 mg

Wheat germ agglutinin (WGA), PEI-coated SPA beads, for proximity-based radiometric scintillation assays. The treatment of PVT-WGA SPA beads with positively charged polyethyleneimine (PEI) blocks potential non-specific binding sites on the SPA bead surface.

For research use only. Not for use in diagnostic procedures.

Feature Specification
Bead Type or Material Polyvinyltoluene (PVT)
Surface Treatment WGA

Wheat germ agglutinin (WGA), PEI-coated SPA beads, for proximity-based radiometric scintillation assays. The treatment of PVT-WGA SPA beads with positively charged polyethyleneimine (PEI) blocks potential non-specific binding sites on the SPA bead surface.

Product Variant
Unit Size: 500 mg
Part #:
SPQ0031
List Price
USD 25,060.00

Overview

Wheat germ agglutinin (WGA), polyvinyltoluene (PVT) PEI-coated SPA beads, for proximity-based radiometric scintillation assays. The treatment of PVT-WGA SPA beads with positively charged polyethyleneimine (PEI) blocks potential non-specific binding sites on the SPA bead surface.

SPA Scintillation beads are microspheres containing scintillant which emit light in the blue region of the visible spectrum. As a result, these beads are ideally suited to use with photomultiplier tube (PMT) counters such as the MicroBeta2 or TopCount.

Two types of core SPA Scintillation bead are available - yttrium silicate (YSi) and Polyvinyltoluene (PVT). PVT beads are plastic, larger in size, and stay in suspension longer than the crystalline YSi beads.

Scintillation proximity assay (SPA) is a homogeneous and versatile technology for the rapid and sensitive assay of a wide range of biological processes, including applications using enzyme and receptor targets, radioimmunoassays, and molecular interactions. When 3H, 14C, 33P, and 125I radioisotopes decay, they release β-particles (or Auger electrons, in the case of 125I). The distance these particles travel through an aqueous solution is dependent on the energy of the particle. If a radioactive molecule is held in close enough proximity to a SPA Scintillation Bead or a SPA Imaging Bead, the decay particles stimulate the scintillant within the bead to emit light, which is then detected in a PMT-based scintillation counter or on a CCD-based imager, respectively. However, if the radioactive molecule does not associate with the SPA bead, the decay particles will not have sufficient energy to reach the bead and no light will be emitted. This discrimination of binding by proximity means that no physical separation of bound and free radiochemical is required.

Specifications

Application
Drug Discovery & Development
Automation Compatible
Yes
Bead Type or Material
Polyvinyltoluene (PVT)
Brand
SPA Scintillation Beads
Detection Modality
Radiometric
Format
Microplates
Tubes
Instrument Compatibility
Microbeta2 counter
Shipping Conditions
Shipped Ambient
Surface Treatment
WGA
Technology
Scintilation Proximity Assay
Unit Size
500 mg

Resources

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Guide
Optimization of SPA receptor binding assays

Scintillation proximity assay has been successfully applied to receptor binding assays

SDS, COAs, Manuals and more

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Safety data sheet
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Technical data sheet
  • Lot number
    ANY
    Lot date
    September 15, 2023
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