Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight™-labeled synthetic peptide containing the amino acid residues surrounding Ser40 of tyrosine hydoxylase. The ULight-tyrosine hydroxylase peptide can be phosphorylated at Ser40 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif: RRQSLIE
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
LANCE Ultra TR-FRET technology provides a two-component kinase assay that combines direct labeling of the kinase substrate with ULight™, an innovative emission dye for optimum binding efficiency, reducing steps and assay prep time. Validated for over 310 kinases, we provide data transparency by supplying:
For ULight substrates:
In LANCE Ultra kinase assays, the binding of a Eu-labeled anti-phosphosubstrate antibody to the phosphorylated ULight-labeled substrate brings donor and acceptor molecules into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye, which in turn generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight-substrate phosphorylation.
Application |
Kinase
|
---|---|
Automation Compatible |
Yes
|
Brand |
LANCE Ultra
|
Detection Method |
TR-FRET
|
Experimental Type |
In vitro
|
Fluorophore |
ULight
|
Format |
Microplates
|
Molecular Modification |
Phosphorylation
|
One Unit Contains |
5.0 nmol
|
Shipping Conditions |
Shipped in Dry Ice
|
Unit Size |
5 nmol
|
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