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LANCE Ultra ACC Ulight Peptide Substrate, 5 nmoles

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide derived from residues 73-85 of rat Acetyl-CoA Carboxylase in which Ser77 is mutated to Ala (also known as SAMS). The ULight-Acetyl-CoA Carboxylase peptide can be phosphorylated at Ser 79 by the AMPKα1 kinase. Peptide sequence: CHMRSAMSGLHLVKRR. Please note this product replaces TRF0118-D or TRF0118-M, now being supplied lyophilized rather than in liquid (frozen) format.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Biochemical Enzymatic Assay

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide derived from residues 73-85 of rat Acetyl-CoA Carboxylase in which Ser77 is mutated to Ala (also known as SAMS). The ULight-Acetyl-CoA Carboxylase peptide can be phosphorylated at Ser 79 by the AMPKα1 kinase. Peptide sequence: CHMRSAMSGLHLVKRR. Please note this product replaces TRF0118-D or TRF0118-M, now being supplied lyophilized rather than in liquid (frozen) format.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 0.5 nmol
Part #:
TRF0133-D
List Price
USD 596.88
Unit Size: 5 nmol
Part #:
TRF0133-M
List Price
USD 1,709.00

Overview

LANCE Ultra TR-FRET technology provides a two-component kinase assay that combines direct labeling of the kinase substrate with ULight™, an innovative emission dye for optimum binding efficiency, reducing steps and assay prep time. Validated for over 310 kinases, we provide data transparency by supplying:

  • Test conditions: Rapid assay setup to move straight into optimized assay performance
  • S:B data: Confidence in proven performance of the kinase on specific substrates; no guessing
  • Phospho-motifs: Substrate sequences are provided
  • Assay options: Multiple substrates are tested so you can select the best peptide sequence and S:B for your kinase

For ULight substrates:

  • 0.5 nmoles = 1,000 assay points
  • 5 nmoles = 10,000 assay points

In LANCE Ultra kinase assays, the binding of a Eu-labeled anti-phosphosubstrate antibody to the phosphorylated ULight-labeled substrate brings donor and acceptor molecules into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye, which in turn generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight-substrate phosphorylation.

Specifications

Application
Biochemical Enzymatic Assay
Automation Compatible
Yes
Brand
LANCE Ultra
Detection Modality
LANCE
Product Group
Fluorescent Reagent
Shipping Conditions
Shipped in Dry Ice
Target Class
Kinases
Technology
TR-FRET
Therapeutic Area
Cardiovascular
Unit Size
5 nmol

Resources

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Guide Icon
Guide
Finding the pathway to assay optimization is easy

Comprehensive Kinase and Antibody Selection for LANCE Ultra Assays

Our comprehensive guide helps you identify and utilize kinases...

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Product Info
LANCE and LANCE Ultra TR-FRET technology product list

LANCE™ Ultra TR-FRET is a no-wash proximity assay technology that combines the benefits of time resolution (TR) with fluorescence...

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