5 mCi quantity of [Methyl-³H]-Thymidine with specific activity of 70-90 Ci is available for your research. Application of [³H]Thymidine can be found in: carboxy-terminal PTH fragments stimulating incorporation in vascular endothelial cells, quantification of granulocyte-macrophage colony-stimulating factor (GM-CSF) hypersensitivity in juvenile myelomonocytic leukemia (JMML), influence of parasympathetic non-adrenergic, non-cholinergic mechanisms on uptake (rat) in response to mastication, measurement of dendritic cell induced T cell response by intracellular ATP levels and uptake in human immunology, etc.

Special Information: If aqueous solutions of thymidine are stored frozen (-20°C), care should be taken to protect the material from U.V. light during the thawing process to prevent possible dimerization.

When [methyl-³H]thymidine is incorporated into cellular DNA and undergoes radiolytic decomposition, [³H]5-hydroxymethyl-2''-deoxyuridine (HMdU) is formed.* The formation of HMdU through transmutation of [methyl-³H]thymidine occurs at the rate of B-decay, which is 0.017% per day. The transmutation product is not volatile.

*References

• S. Apelgot and B. Ekert, J. Chem. Phys. 60, 505 (1963).
• K. Fink, R. E. Cline, and R. M. Fink, Anal. Chem., 35, 389 (1963).
• Specifications and Criteria for Biochemical Compounds, Third Edition (ISBN 0-309-01917-6), National Academy of Sciences - National Research Council, Washington, D.C. 1972.
• G. W. Teebor, K. Frenkel, and M. S. Goldstein, Proc. Natl. Acad. Sci. USA 81, 318 (1984).
• H. Tono, S.S. Cohen, J. Biol. Chem. 237, 1271 (1962).
• M. Nemer, J. Biol. Chem. 237, 143 (1962).