APC-labeled streptavidin, for use in LANCE® TR-FRET protein binding assays.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Protein-Protein Interaction |
APC-labeled streptavidin, for use in LANCE® TR-FRET protein binding assays.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Revvity offers two versions of SureLight APC-SA: one protein binding assays, and one kinase assays. SureLight APC-SA protein binding assays has a ratio of APC:streptavidin that has been optimized to achieve best performance in protein binding applications.
LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from donor Europium chelate to acceptor fluorophore if sufficiently close FRET (~10 nm). This results in emission of light at 665 nm (ULight dye and APC).
Application |
Protein-Protein Interaction
|
---|---|
Automation Compatible |
Yes
|
Brand |
LANCE SureLight
|
Conjugates |
Streptavidin
|
Detection Modality |
LANCE
|
Fluorophore |
SureLight APC
|
Product Group |
Fluorescent Reagent
|
Shipping Conditions |
Shipped in Blue Ice
|
Target Class |
Binding Assay
|
Technology |
TR-FRET
|
Unit Size |
1 mg
|
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LANCE™ Ultra TR-FRET is a no-wash proximity assay technology that combines the benefits of time resolution (TR) with fluorescence...
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