APC-labeled streptavidin, for use in LANCE® TR-FRET protein binding assays.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Revvity offers two versions of SureLight APC-SA: one protein binding assays, and one kinase assays. SureLight APC-SA protein binding assays has a ratio of APC:streptavidin that has been optimized to achieve best performance in protein binding applications.
LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from donor Europium chelate to acceptor fluorophore if sufficiently close FRET (~10 nm). This results in emission of light at 665 nm (ULight dye and APC).
Antibody Conjugates |
Streptavidin
|
---|---|
Application |
Protein-protein interactions
Protein Analysis & Detection
|
Automation Compatible |
Yes
|
Brand |
LANCE SureLight
|
Detection Method |
Time-Resolved Fluorescence (TRF)
TR-FRET
|
Experimental Type |
In vitro
|
Fluorophore |
SureLight APC
|
Format |
Microplates
|
Unit Size |
30 mg
|
Here, we demonstrate the utility and benefits of using LANCE Ultra TR-FRET assay technology for identifying and characterizing PD...
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