Positive control lysate for AlphaLISA™ SureFire® Ultra™ Multiplex Phospho/Total NFκB Detection Kit.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
The positive control lysate is prepared from HeLa cells, cultured to confluence in T175 flasks in 10% FBS containing medium, then treated with 50ng/mL TNFα and 20ng/mL Calyculin A for 10 minutes and lysed in 10mL of 1X SureFire Ultra Lysis buffer.
Application |
Protein Detection
|
---|---|
Assay Target |
NFkb
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra Multiplex
|
Detection Method |
Alpha
|
Shipping Conditions |
Shipped in Blue Ice
|
Unit Size |
1 vial
|
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