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AlphaLISA SureFire Ultra Multiplex Human Phospho-NF-κB (Ser536 & Total) Detection Kit, Control Lysate

Positive control lysate for AlphaLISA™ SureFire® Ultra™ Multiplex Phospho/Total NFκB Detection Kit.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Part number: MPSU-PTNFKB-K-L
List price: USD 142.66
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USD 142.66
USD 142.66 /each

Overview

The positive control lysate is prepared from HeLa cells, cultured to confluence in T175 flasks in 10% FBS containing medium, then treated with 50ng/mL TNFα and 20ng/mL Calyculin A for 10 minutes and lysed in 10mL of 1X SureFire Ultra Lysis buffer.

Specifications

Application
Protein Detection
Assay Target
NFkb
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra Multiplex
Detection Method
Alpha
Shipping Conditions
Shipped in Blue Ice
Unit Size
1 vial

Video gallery

Resources

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Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

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Guide
AlphaPlex quick start guide

The AlphaPlex™ reagent system was designed to enable fast and easy transition from well-established AlphaLISA™ assays to...

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