Positive control lysate for AlphaLISA™ SureFire® Ultra™ Multiplex Phospho/Total NFκB Detection Kit.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Protein Quantification |
Positive control lysate for AlphaLISA™ SureFire® Ultra™ Multiplex Phospho/Total NFκB Detection Kit.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
The positive control lysate is prepared from HeLa cells, cultured to confluence in T175 flasks in 10% FBS containing medium, then treated with 50ng/mL TNFα and 20ng/mL Calyculin A for 10 minutes and lysed in 10mL of 1X SureFire Ultra Lysis buffer.
Application |
Protein Quantification
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra Multiplex
|
Detection Modality |
Alpha
|
Lysis Buffer Compatibility |
Lysis Buffer
|
Molecular Modification |
Phosphorylation
|
Product Group |
Control Lysate
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
NFkb
|
Target Class |
Phosphoproteins
|
Target Species |
Human
|
Technology |
Alpha
|
Unit Size |
1 Vial
|
Are you looking for resources, click on the resource type to explore further.
This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
The AlphaPlex™ reagent system was designed to enable fast and easy transition from well-established AlphaLISA™ assays to...
We are here to answer your questions.