Positive control lysate for AlphaLISA™ SureFire® Ultra™ Multiplex Phospho/Total NFκB Detection Kit.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
The positive control lysate is prepared from HeLa cells, cultured to confluence in T175 flasks in 10% FBS containing medium, then treated with 50ng/mL TNFα and 20ng/mL Calyculin A for 10 minutes and lysed in 10mL of 1X SureFire Ultra Lysis buffer.
Application |
Protein Detection
|
---|---|
Assay Target |
NFkb
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra Multiplex
|
Detection Method |
Alpha
|
Shipping Conditions |
Shipped in Blue Ice
|
Unit Size |
1 vial
|
Now you can quantify multiple analytes in a single well for faster and more accurate results, even with low sample volumes
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