Positive control lysate for AlphaLISA™ SureFire® Ultra™ Multiplex Phospho/Total ErbB2 Detection Kit.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Protein Quantification |
Positive control lysate for AlphaLISA™ SureFire® Ultra™ Multiplex Phospho/Total ErbB2 Detection Kit.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
The positive control lysate is prepared from A431 cells, cultured in a T175 flask in 10% FBS containing medium, starved overnight, then treated with 1μg/mL EGF for 5min and lysed in 4mL of 1X SureFire Ultra Lysis buffer.
Application |
Protein Quantification
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra Multiplex
|
Detection Modality |
Alpha
|
Lysis Buffer Compatibility |
Lysis Buffer
|
Molecular Modification |
Phosphorylation
|
Product Group |
Control Lysate
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
ErbB2
|
Target Class |
Phosphoproteins
|
Target Species |
Human
|
Technology |
Alpha
|
Unit Size |
1 Vial
|
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