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Multiplex Surefire Ultra p-EGFR (Tyr1068) (Eu) + Total EGFR (Tb) Kit, 10,000 Assay Points
The Alpha SureFire® Ultra™ Multiplex p-EGFR (Tyr1068) + Total EGFR assay kit is used to measure both phosphorylated and total levels of EGFR in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure EGFR (Tyr1068) activation by either recombinant or endogenous receptors, and can be applied to primary cells.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product information
Overview
The Alpha SureFire Ultra Multiplex p-EGFR + Total EGFR assay kit is used to measure both phosphorylated and total levels of endogenous EGFR in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated EGFR analysis, and the 545 nm (Tb) signal corresponds to the total EGFR analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.
- No-wash steps, no separation steps
- Multiplexing assay
- Sensitive detection
- No interference from exogenous antibodies
- Results in less than 3 hours
In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.
Specifications
| Application |
Kinase
Multiplexing
Protein Analysis & Detection
|
|---|---|
| Assay Points |
10000
|
| Assay Target Class |
Phospho-protein
Protein
|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra Multiplex
|
| Detection Method |
Alpha
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Unit Size |
10,000 Assay Points
|
Image gallery
Video gallery
Resources
This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
This Quick Start Guide is intended to provide the Alpha user with the knowledge and tools necessary to quickly and easily unleash...
Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...
Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
Now you can quantify multiple analytes in a single well for faster and more accurate results, even with low sample volumes
SDS, COAs, Manuals and more
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