The Alpha SureFire® Ultra™ Multiplex phospho Cofilin (Ser3) + Total Cofilin assay kit is used to measure both the phosphorylation (on Ser3) and total levels of endogenous Cofilin in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Cofilin phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
The Alpha SureFire Ultra Multiplex p-Cofilin (Ser3) + Total Cofilin assay kit is used to measure both phosphorylated and total levels of endogenous Cofilin in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated Cofilin analysis, and the 545 nm (Tb) signal corresponds to the total Cofilin analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.
In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.
Application |
Protein Analysis & Detection
|
---|---|
Assay Points |
500
|
Assay Target |
Cofilin
|
Assay Target Class |
Phospho-protein
Protein
|
Assay Technology |
Alpha
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra Multiplex
|
Detection Method |
Alpha
|
Experimental Type |
In vitro
|
Shipping Conditions |
Shipped in Blue Ice
|
Therapeutic Area |
Central Nervous System
|
Unit Size |
500 assay points
|
This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
The AlphaPlex™ reagent system was designed to enable fast and easy transition from well-established AlphaLISA™ assays to...
Emerging pathways to neuroinflammation and neurodegeneration
Neurodegenerative diseases, such as amyotrophic lateral sclerosis...
Are you looking for technical documents related to the product? We have categorized them in dedicated sections below. Explore now.
We are here to answer your questions.