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AlphaLISA SureFire Ultra Multiplex Human Phospho-AMPKα 1/2 (Thr172 & Total) Detection Kit, 50,000 Assay Points
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AlphaLISA SureFire Ultra Multiplex Human Phospho-AMPKα 1/2 (Thr172 & Total) Detection Kit, 50,000 Assay Points
The Alpha SureFire® Ultra™ Multiplex phospho AMPKα1/2 (Thr172) + Total AMPKα1/2 assay kit is used to measure both the phosphorylation (on Thr172) and total levels of endogenous AMPKα1/2 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure AMPKα1/2 phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Sample Volume | 10 µL |
The Alpha SureFire® Ultra™ Multiplex phospho AMPKα1/2 (Thr172) + Total AMPKα1/2 assay kit is used to measure both the phosphorylation (on Thr172) and total levels of endogenous AMPKα1/2 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure AMPKα1/2 phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA SureFire Ultra Multiplex Human Phospho-AMPKα 1/2 (Thr172 & Total) Detection Kit, 50,000 Assay Points
AlphaLISA SureFire Ultra Multiplex Human Phospho-AMPKα 1/2 (Thr172 & Total) Detection Kit, 50,000 Assay Points
Product information
Overview
The Alpha SureFire Ultra Multiplex p-AMPKα1/2 (Thr172) + Total AMPKα1/2 assay kit is used to measure both phosphorylated and total levels of endogenous AMPKα1/2 in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated AMPKα1/2 analysis, and the 545 nm (Tb) signal corresponds to the total Akt1 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.
- No-wash steps, no separation steps
- Multiplexing assay
- Sensitive detection
- No interference from exogenous antibodies
- Results in less than 3 hours
In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra Multiplex
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
AMPKα1/2
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Metabolic
|
| Unit Size |
50,000 Assay Points
|
Image gallery
AlphaLISA SureFire Ultra Multiplex Human Phospho-AMPKα 1/2 (Thr172 & Total) Detection Kit, 50,000 Assay Points
AlphaLISA SureFire Ultra Multiplex Human Phospho-AMPKα 1/2 (Thr172 & Total) Detection Kit, 50,000 Assay Points
Video gallery
AlphaLISA SureFire Ultra Multiplex Human Phospho-AMPKα 1/2 (Thr172 & Total) Detection Kit, 50,000 Assay Points
AlphaLISA SureFire Ultra Multiplex Human Phospho-AMPKα 1/2 (Thr172 & Total) Detection Kit, 50,000 Assay Points
Resources
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Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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