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    • Academia
      Explore BioLegend

      Learn about our world class antibodies for a diverse set of research areas including immunology, neuroscience, cancer, stem cells and cell biology.

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  • Products
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      • Genomic Analysis
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        • CRISPR Technologies
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      • Protein Analysis
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      • Cell Analysis
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      • Research Solutions
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    • Clinical & Diagnostics
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        • Molecular Cytogenetics
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      • Infectious Diseases
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        • EUROIMMUN Solutions
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        • Nucleic Acid Isolation for Pathogen Detection
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        • Metagenomics
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      • Cancer
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        • ctDNA Workflows
        • miRNA-seq Analysis
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        • Mimix Reference Standards
        • Functional Testing
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        Providing diagnostic labs with trusted quality controls for developing tests and monitoring workflows, with the added assurance of IVD marking. Explore our newest MimixTM controls.

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      • Autoimmunity
        • EUROIMMUN Solutions
        • IDS Solutions
        New IVD Mimix™ reference standards for oncology workflows.

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      • Endocrinology
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      • Allergy
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      • Neurodegeneration
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        • Neurogranin
        • BACE1
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      • Rapid Patient Testing
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      New IVD Mimix™ reference standards for oncology workflows.

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    • Reagents
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      • chemagic Nucleic Acid Purification Kits
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      • CRISPR Technologies
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    • Platforms & Automation
      • Nucleic Acid Purification
        • chemagic Applications
        • chemagic IVD Instruments
        • chemagic IVD Kits
        • chemagic Instruments
        • chemagic Kits
        • M-PVA Magnetic Beads
        New! Cellometer™ Ascend™

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      • Automated Liquid Handling
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        • Liquid Handling Consumables & Accessories
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      • Integrated Lab Automation
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        • Robotics
        New! Cellometer™ Ascend™

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      • Microfluidic Analysis
        • Microfluidic Protein Characterization
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        New! Cellometer™ Ascend™

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      • Detection Solutions
        • Plate Reader Accessories
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        New! Cellometer™ Ascend™

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      • Imaging
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        New! Cellometer™ Ascend™

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      • Cell Counting and Image Cytometry
        New! Cellometer™ Ascend™

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      • Sample Homogenization
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        New! Cellometer™ Ascend™

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      • In Vitro Diagnostics (IVD) Platforms & Automation
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        • EUROIMMUN Instruments
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      • Consumables & Accessories
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      • Instrument Service & Maintenance
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      • All Solutions
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  • Services
    • Preclinical Services
      • Antibody Drug Conjugate Services
        Preclinical services

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      • Complex Cell Model Screening Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Base Editing Platform
        • Pin-point base editing platform cell line engineering services
        • Pin-point base editing platform pooled tiled screening services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Immune Cell Screening
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      • Functional Genomic Screening Services
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      • Cell Panel Screening
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      • Cell Line Engineering
        • CHOSOURCE CLE Services
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      • Viral Vector Engineering and Manufacture
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      Preclinical services

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    • Revvity Omics Services
      • Revvity Omics Clinical Services
        • Cytogenomics
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        • Specialized and Customized Assays
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        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Revvity Omics Pharma Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Clinical & Testing Services
      • Revvity Omics Clinical Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Revvity Omics Pharma Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Cellular and Humoral Immunoassays
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Tuberculosis Testing Services
        • Oxford Diagnostic Laboratories Service Closures
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Customization Services
      • Assays and Reagents
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        • Compound profiling
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Microplate Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Custom Conjugation & Labeling
        • Custom Labeling
        • Small Molecule
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Radiosynthesis and Labeling
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Licensing
      • Gene Delivery Licensing
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      • Gene Expression Systems
        • CHOSOURCE Platform
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      • Pin-point™ Base Editing Platform
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      • Virus Screening
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      CHOSOURCE expression platform

      Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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    • Viral Vector Engineering and Manufacture
      • AAV Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Lentivirus Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Instrument Service & Maintenance
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        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Equipment Service Plans
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • On-demand Equipment Service
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Customer Training
      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • OEM Solutions
      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    T-SPOT.TB testing services.

    Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Popular Revvity Product Brands
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    • BioQule NGS

    • chemagic

    • EnVision Nexus

    • Fontus

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    • IVIS

    • LEGENDplex

    • Opera Phenix

    • T-SPOT

    • TotalSeq

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  • Welcome to Revvity: renowned brands and boundless innovation.

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    Learn about our world-class antibodies for a diverse set of research areas including immunology, neuroscience, cancer, stem cells and cell biology.

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Cellaca PLX High-throughput Image Cytometer

Knowledge base

The Cellaca™ PLX is a high-throughput image cytometer for cell analysis workflows, delivering fast cell counts (up to 24 samples in <5 mins), concentration, and viability readouts. Additionally, the Cellaca PLX can detect surface markers, apoptotic markers, and transduction and transfection efficiency – all at the lab bench.

For research use only. Not for use in diagnostic procedures.
Part #: PLX-SYS1
Imaging Modality:
Fluorescence, Brightfield
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Imaging Modality: Fluorescence, Brightfield
Part #: PLX-SYS1
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Overview

The Cellaca PLX image cytometer provides versatile cell analysis ranging from simple cell counts, concentration, and viability readouts, to more complex multiplexed assays, all using only 15-50 µL of sample. The sensitivity of the system allows for the analysis of up to 24 samples using dual fluorescence and brightfield imaging in under 5 minutes, and single sample multiplexing with four channels in 1 minute.

Multiplex panels for immunophenotyping, apoptosis, and fluorescent protein expression shorten the time to downstream processing with Cellaca PLX low fluorescence slides, dedicated reagents, built-in assays, and configurable data reports. The Cellaca PLX features user friendly software, automation compatibility, and is 21 CFR Part 11 ready. Expert support is provided to integrate the system into current workflows to streamline cell line development, cell isolation, gene delivery, and various research applications.

Key features and benefits of the Cellaca PLX:

  • Elevated throughput - run up to 24 samples in under 5 minutes using dual fluorescence and brightfield imaging to count both isolated nuclei and cells
  • Multiplexing capabilities - shorten time to downstream processing (one minute per sample with four channels) using our custom reagents kits for surface marker, apoptosis, and fluorescent protein analysis
  • Small sample volume - as little as 15 µL of cell sample needed
  • Intuitive workflows - including a favorites feature for saving customized experiments and commonly used assays with automatic data analysis and reporting
  • Automation ready - robotic integration ability with via API
  • 21 CFR Part 11 ready - add-on that includes audit trail, user access control, and digital signature

Additional product information

Cellaca PLX applications

With the Cellaca PLX platform, you can:

  • Quickly perform cell purity checks and viability at the bench with Cellaca PLX immunophenotyping kits which combine CD3, CD4, and CD8 surface marker detection with cell viability in a single multiplex assay.
  • Stain transfected cell lines with viability dyes to determine the percentage of transfected or transduced cells that are live/dead.
  • Perform routine viability measurements as well as apoptosis functional assays to determine cell health.

Cellaca PLX optimized assay reagents and kits

The Cellaca PLX system provides flow-like data with simple-to-use, mix, incubate, wash, and read reagent kits that provide immunophenotyping plus cell counts and viability multiplexing assays using disposable, low fluorescence consumables. Quickly perform cell counts, concentration, purity checks, and viability at the bench with Cellaca PLX immunophenotyping kits which combine CD3, CD4, and CD8 surface marker detection with cell viability in a single multiplex assay.

Available assay kits include:

  • Immune cell phenotyping
  • Apoptosis detection
  • High-throughput cell viability
  • Fluorescent protein analysis

Low auto-fluorescent consumables

Cellaca PLX consumable slides provide a safe and self-contained environment for the sample which then does not require a decontamination procedure of the instrument at the end of each run. The slide holder, which is included with the instrument, can accommodate 4 slides or 8 samples per experimental run.

Software that makes multiplexing easy

Stress-free Cellaca PLX software comes with preloaded, step-by-step templates and methodologies to streamline multiplex analysis, providing a benchtop solution for quick, reliable measurements of cell counts and concentrations, surface marker staining, viability, and apoptosis. And you get customizable results for presentations, too. 

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A Cellaca instrument for every analysis

The Cellaca instrument line comprises the Cellaca MX high-throughput cell counter, with brightfield and two fluorescent channels, and the Cellaca PLX image cytometry system, with brightfield and five fluorescent channels, for multichannel analysis.

  Cellaca PLX Cellaca MX (MX-AOPI)
Channels Brightfield, Blue, Green, Red, Far Red Brightfield, Green, Red
Number of fluorescent channels 13 (6 per scan) 3 (2 per scan)
Excitation LED 370 nm, 475 nm, 531 nm and 628 nm 470 nm, 527nm
Emissions Filters 452 nm, 534 nm, 605 nm, 655 nm and 692 nm 534 nm, 655 nm
Commonly Used Compatible Dyes Trypan Blue, AO/PI, Hoechst, DAPI, GFP, RFP, CMFDA, Calcein AM, 7AAD, Annexin V, PE, APC, KIRAVIA Blue 520™* Trypan Blue, AO/PI, Calcein AM, Annexin V, Caspase 3/7
Counting Speed Fluorescence 4-channel scan in
1 minute (per plate)
Trypan Blue - 2 seconds
Fluorescence 2-channel scan-
Less than 8 seconds (per well)
Volume (per well) 15 µL in slides
50 µL - 200 µL in counting plates
50 µL - 200 µL in counting plates
Size/Diameter Range 5 µm - 80 µm 5 µm - 80 µm
Concentration Range 1x105 - 1x107 cell/mL 1x105 - 1x107 cell/mL
IQ/OQ Option Yes Yes
21 CFR Part 11 Ready Option  Yes Yes
Automation Compatible Yes Yes
Dimensions 13 in x 13 in x 16 in
(33 cm x 33 cm x 41 cm)
13 in x 13 in x 16 in
(33 cm x 33 cm x 41 cm)
Weight 42 lbs (19 kg) 42 lbs (19 kg)

Specifications

Imaging Modality
Fluorescence, Brightfield
Unit Size
1 unit

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Cellaca PLX High-throughput Image Cytometer
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Cellaca PLX High-throughput Image Cytometer
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Application Note
Novel high-throughput image cytometry method for T cell immunophenotyping and viability readouts

Key features of our Cellaca PLX application note:

  • High-throughput and rapid cell concentration analysis with sample efficient...
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