PhenoVue Nile Red Lipid stain is a lipophilic organic molecule commonly used for localization and quantification of intracellular lipid droplets.
PhenoVue Nile Red Lipid stain exhibits bright yellow fluorescence and is validated for use in imaging microscopy and high-content screening applications.
PhenoVue Nile Red Lipid stain exhibits:
View our extensive validation data in the Product Information Sheet within the Resources tab below.
For research use only. Not for use in diagnostic procedures.
Feature | Specification |
---|---|
Color | Yellow/Orange |
Filter | Cy3 |
Organelle and Cell Compartment | Lipid droplets |
PhenoVue Nile Red Lipid stain is a lipophilic organic molecule commonly used for localization and quantification of intracellular lipid droplets.
PhenoVue Nile Red Lipid stain exhibits bright yellow fluorescence and is validated for use in imaging microscopy and high-content screening applications.
PhenoVue Nile Red Lipid stain exhibits:
View our extensive validation data in the Product Information Sheet within the Resources tab below.
PhenoVue Nile Red Lipid stain is a lipophilic organic molecule that is almost nonfluorescent in water and polar solvents. In lipid-rich environments, Nile Red exhibits enhanced yellow fluorescence, as well as red fluorescence to a lesser extent.
Nile Red is commonly used for localization and quantification of intracellular lipid droplets which are involved in lipid synthesis, metabolism and transportation as well as protein storage and degradation or viral replication, all of which are related to pathophysiology, including dyslipidemia, obesity, lipodystrophy, diabetes, fatty liver diseases, or atherosclerosis.
Numbers of vials per unit | 2 |
---|---|
Quantity or volume per vial | 10mg (31.4µmoles) |
Form | Powder |
Storage | -20°C |
Recommended working concentration | 200nM (0.008ng/mL) |
Maximum excitation wavelength | 512 nm - complexed with triglycerides 552 nm - complexed with phospholipids* (*as shown in SpectraViewer below) |
Maximum emission wavelength | 585 nm - complexed with triglycerides 638 nm - complexed with phospholipids* (*as shown in SpectraViewer below) |
Common filter set | Cy3.5 |
Live cell staining | Yes |
Fixed cell staining | Yes. See Product Information Sheet for more information |
Equivalent number of microplates | 10900-32700 x 96-well microplates 9090-32700 x 384-well microplates 17040-40900 x 1536-well microplates |
Color |
Yellow/Orange
|
---|---|
Form |
Powder
|
Maximum Emission Wavelength (Emmax) |
Triglycerides: 585 nm, phospholipids: 638 nm
|
Maximum Excitation Wavelength (Exmax) |
Triglycerides: 512 nm, phospholipids: 552 nm
|
Application |
High Content Imaging
Microscopy
|
---|---|
Brand |
PhenoVue™
|
Detection Modality |
Fluorescence
|
Filter |
Cy3
|
Organelle and Cell Compartment |
Lipid droplets
|
Quantity |
2 x 10 mg
|
Sample Type |
Live and fixed samples
|
Shipping Conditions |
Shipped Ambient
|
Storage Conditions |
Room temperature or 2-8 °C, protected from light
|
Type |
Individual Reagent
|
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This is a product information sheet for PhenoVue Nile Red Lipid Stain.
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