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PhenoVue Fluor 647 - Goat Anti-Rabbit Antibody Highly Cross-Adsorbed

PhenoVue Fluor 647 - Goat Anti-Rabbit Antibody, Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 555 - Goat Anti-Mouse IgG Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 555 - Goat Anti-Mouse IgG (H+L) Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 555 - Goat Anti-Mouse IgG Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 555 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 555 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange) + PhenoVue Hoechst 33342 (nucleus, blue). Imaged on Operetta CLS.
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 555 Goat Anti-Mouse IgG (H+L) Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Mouse IgG Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Rabbit IgG Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Rabbit IgG Highly Cross-Adsorbed
PhenoVue Fluor 647 - Goat Anti-Rabbit Antibody, Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 555 - Goat Anti-Mouse IgG Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 555 - Goat Anti-Mouse IgG (H+L) Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 555 - Goat Anti-Mouse IgG Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 555 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 555 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange) + PhenoVue Hoechst 33342 (nucleus, blue). Imaged on Operetta CLS.
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 555 Goat Anti-Mouse IgG (H+L) Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Mouse IgG Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Rabbit IgG Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Rabbit IgG Highly Cross-Adsorbed
PhenoVue Fluor 647 - Goat Anti-Rabbit Antibody, Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 555 - Goat Anti-Mouse IgG Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 555 - Goat Anti-Mouse IgG (H+L) Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 555 - Goat Anti-Mouse IgG Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 555 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 555 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange) + PhenoVue Hoechst 33342 (nucleus, blue). Imaged on Operetta CLS.
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 555 Goat Anti-Mouse IgG (H+L) Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Mouse IgG Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Rabbit IgG Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Rabbit IgG Highly Cross-Adsorbed
PhenoVue Fluor 647 - Goat Anti-Rabbit Antibody, Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 555 - Goat Anti-Mouse IgG Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 555 - Goat Anti-Mouse IgG (H+L) Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 555 - Goat Anti-Mouse IgG Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 555 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 555 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange) + PhenoVue Hoechst 33342 (nucleus, blue). Imaged on Operetta CLS.
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 555 Goat Anti-Mouse IgG (H+L) Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Mouse IgG Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Rabbit IgG Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Rabbit IgG Highly Cross-Adsorbed

Highly cross-adsorbed goat anti-rabbit IgG (H+L) antibody conjugated with PhenoVue™ Fluor 647.

PhenoVue Fluor 647 secondary antibody conjugates exhibit bright red-orange fluorescence and are validated for use in high content screening applications.

Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue Fluor - 647 goat anti-rabbit antibody, highly cross-adsorbed, has a maximum excitation wavelength of 650nm and a maximum emission wavelength of 670nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only, not for use in diagnostic procedures.

Product information SDS, COAs, Manuals and more
Part number: 2GXRB647H1
List price: USD 264.69
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USD 264.69
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Product information

Overview

PhenoVue Fluor 647 - goat anti-rabbit IgG (H+L) antibody, highly cross-adsorbed, has been cross-adsorbed against various IgG species such as rat, mouse, hamster, human, bovine, horse, or chicken serum to minimize cross-reactivity with other species.

Specificity and sensitivity are improved and background reduced with PhenoVue Fluor 647 - goat anti-Rabbit IgG (H+L) antibody, highly cross-adsorbed.

It can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.

Additional product information

Features
Numbers of vials per unit 1
Quantity or volume per unit 1 mg
Form Lyophilized
Storage 2-8°C
Recommended working concentration 1.5 µg/mL(10nM)
Maximum excitation wavelength 650nm
Maximum emission wavelength 670nm
Common filter set Cy5
Live cell staining No
Fixed cell staining Yes
Equivalent number of microplates 25-70 x 96-well microplates
20-70 x 384-well microplates
35-90 x 1536-well microplates

 

Specifications

Color
Red
Brand
PhenoVue™
Detection Method
Fluorescence
Filter
Cy5
Fluorophore
PhenoVue™ Fluor 647
Host
Goat
Product Compatibility
Fixed samples only
Target Species
Rabbit
Type
Individual Reagent
Unit Size
1 vial

Image gallery

PhenoVue Fluor 647 - Goat Anti-Rabbit Antibody, Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 555 - Goat Anti-Mouse IgG Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 555 - Goat Anti-Mouse IgG (H+L) Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 555 - Goat Anti-Mouse IgG Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 555 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 555 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange) + PhenoVue Hoechst 33342 (nucleus, blue). Imaged on Operetta CLS.
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 555 Goat Anti-Mouse IgG (H+L) Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Mouse IgG Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Rabbit IgG Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Rabbit IgG Highly Cross-Adsorbed
PhenoVue Fluor 647 - Goat Anti-Rabbit Antibody, Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 555 - Goat Anti-Mouse IgG Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 555 - Goat Anti-Mouse IgG (H+L) Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 555 - Goat Anti-Mouse IgG Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 555 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 555 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange) + PhenoVue Hoechst 33342 (nucleus, blue). Imaged on Operetta CLS.
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 555 Goat Anti-Mouse IgG (H+L) Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Mouse IgG Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Rabbit IgG Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Rabbit IgG Highly Cross-Adsorbed
PhenoVue Fluor 647 - Goat Anti-Rabbit Antibody, Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 555 - Goat Anti-Mouse IgG Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 555 - Goat Anti-Mouse IgG (H+L) Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 555 - Goat Anti-Mouse IgG Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 555 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 555 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange) + PhenoVue Hoechst 33342 (nucleus, blue). Imaged on Operetta CLS.
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 555 Goat Anti-Mouse IgG (H+L) Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Mouse IgG Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Rabbit IgG Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Rabbit IgG Highly Cross-Adsorbed
PhenoVue Fluor 647 - Goat Anti-Rabbit Antibody, Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 555 - Goat Anti-Mouse IgG Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 555 - Goat Anti-Mouse IgG (H+L) Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 555 - Goat Anti-Mouse IgG Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 555 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 555 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange) + PhenoVue Hoechst 33342 (nucleus, blue). Imaged on Operetta CLS.
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 555 Goat Anti-Mouse IgG (H+L) Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Mouse IgG Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Rabbit IgG Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Rabbit IgG Highly Cross-Adsorbed

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