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PhenoVue Fluor 647 - Goat Anti-Rabbit Antibody Cross-Adsorbed

SDS COAs
PhenoVue Fluor 647 - Goat Anti-Rabbit Antibody, Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 647 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange) + PhenoVue Hoechst 33342 (nucleus, blue). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 647 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange). Imaged on Operetta CLS.
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 647 - Goat Anti-Mouse IgG Cross-Adsorbed and Highly Cross- Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Mouse IgG Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Rabbit IgG Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 - Goat Anti-Mouse IgG Highly Cross-Adsorbed
PhenoVue Fluor 647 - Goat Anti-Rabbit Antibody, Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 647 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange) + PhenoVue Hoechst 33342 (nucleus, blue). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 647 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange). Imaged on Operetta CLS.
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 647 - Goat Anti-Mouse IgG Cross-Adsorbed and Highly Cross- Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Mouse IgG Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Rabbit IgG Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 - Goat Anti-Mouse IgG Highly Cross-Adsorbed
PhenoVue Fluor 647 - Goat Anti-Rabbit Antibody, Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 647 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange) + PhenoVue Hoechst 33342 (nucleus, blue). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 647 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange). Imaged on Operetta CLS.
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 647 - Goat Anti-Mouse IgG Cross-Adsorbed and Highly Cross- Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Mouse IgG Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Rabbit IgG Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 - Goat Anti-Mouse IgG Highly Cross-Adsorbed
PhenoVue Fluor 647 - Goat Anti-Rabbit Antibody, Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 647 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange) + PhenoVue Hoechst 33342 (nucleus, blue). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 647 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange). Imaged on Operetta CLS.
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 647 - Goat Anti-Mouse IgG Cross-Adsorbed and Highly Cross- Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Mouse IgG Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Rabbit IgG Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 - Goat Anti-Mouse IgG Highly Cross-Adsorbed

Cross-adsorbed goat anti-rabbit IgG (H+L) antibody conjugated with PhenoVue™ Fluor 647.

PhenoVue Fluor 647 secondary antibody conjugates exhibit bright red-orange fluorescence and are validated for use in high-content screening applications.

Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue Fluor - 647 goat anti-rabbit antibody, cross-adsorbed, has a maximum excitation wavelength of 650nm and a maximum emission wavelength of 670nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

Product information

For research use only, not for use in diagnostic procedures.

SDS COAs
Part number: 2GXRB647C1
List price: USD 214.87
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USD 214.87
USD 214.87 /each
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Product information

Overview

PhenoVue Fluor 647 - goat anti-rabbit IgG (H+L) antibody, cross-adsorbed, is affinity purified and recognizes Rabbit IgG.

It can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.

PhenoVue Fluor 647 - goat anti-rabbit IgG (H+L) antibody, cross-adsorbed, may display cross-reactivity with other species such as human or Guinea pig. For improved specificity and lower background, or in multiplexed applications, consider using highly cross-adsorbed PhenoVue Fluor conjugated secondary antibodies as an alternative.

Additional product information

Features
Numbers of vials per unit 1
Quantity or volume per unit 1 mg
Form Lyophilized
Storage 2-8°C
Recommended working concentration 1.5µg/mL (10nM)
Maximum excitation wavelength 650nm
Maximum emission wavelength 670nm
Common filter set Cy5
Live cell staining No
Fixed cell staining Yes
Equivalent number of microplates 25-70 x 96-well microplates
20-70 x 384-well microplates
35-90 x 1536-well microplates

 

Specifications

Color
Red
Brand
PhenoVue™
Detection Method
Fluorescence
Filter
Cy5
Fluorophore
PhenoVue™ Fluor 647
Host
Goat
Product Compatibility
Fixed samples only
Shipping Conditions
Shipped Ambient
Target Species
Rabbit
Type
Individual Reagent
Unit Size
1 vial

Image gallery

PhenoVue Fluor 647 - Goat Anti-Rabbit Antibody, Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 647 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange) + PhenoVue Hoechst 33342 (nucleus, blue). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 647 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange). Imaged on Operetta CLS.
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 647 - Goat Anti-Mouse IgG Cross-Adsorbed and Highly Cross- Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Mouse IgG Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Rabbit IgG Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 - Goat Anti-Mouse IgG Highly Cross-Adsorbed
PhenoVue Fluor 647 - Goat Anti-Rabbit Antibody, Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 647 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange) + PhenoVue Hoechst 33342 (nucleus, blue). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 647 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange). Imaged on Operetta CLS.
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 647 - Goat Anti-Mouse IgG Cross-Adsorbed and Highly Cross- Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Mouse IgG Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Rabbit IgG Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 - Goat Anti-Mouse IgG Highly Cross-Adsorbed
PhenoVue Fluor 647 - Goat Anti-Rabbit Antibody, Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 647 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange) + PhenoVue Hoechst 33342 (nucleus, blue). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 647 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange). Imaged on Operetta CLS.
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 647 - Goat Anti-Mouse IgG Cross-Adsorbed and Highly Cross- Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Mouse IgG Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Rabbit IgG Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 - Goat Anti-Mouse IgG Highly Cross-Adsorbed
PhenoVue Fluor 647 - Goat Anti-Rabbit Antibody, Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 647 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange) + PhenoVue Hoechst 33342 (nucleus, blue). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 647 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange). Imaged on Operetta CLS.
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 647 - Goat Anti-Mouse IgG Cross-Adsorbed and Highly Cross- Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Mouse IgG Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 Goat Anti-Rabbit IgG Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 - Goat Anti-Mouse IgG Highly Cross-Adsorbed

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Resources

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PhenoVue Fluor - Goat Anti-Rabbit Secondary Antibodies Product Information Sheet

This is a product information sheet for PhenoVue Fluor - Goat anti-Rabbit Secondary Antibodies.

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