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PhenoVue Fluor 647 - Donkey Anti-Rabbit Antibody Cross-Adsorbed
Cross-adsorbed donkey anti-rabbit IgG (H+L) antibody conjugated with PhenoVue™ Fluor 647.
PhenoVue Fluor 647 secondary antibody conjugates exhibit bright red-orange fluorescence and are validated for use in high-content screening applications.
Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue Fluor - 647 donkey anti-rabbit antibody, cross-adsorbed, has a maximum excitation wavelength of 650nm and a maximum emission wavelength of 670nm.
View our extensive validation data in the Product Information Sheet within the Resources tab below.
For research use only, not for use in diagnostic procedures.
Product information
Overview
PhenoVue Fluor 647 - donkey anti-rabbit IgG (H+L) antibody, cross-adsorbed, is affinity purified and recognizes rabbit IgG. It can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
PhenoVue Fluor 647 - donkey anti-rabbit IgG (H+L) antibody, cross-adsorbed, may display cross-reactivity with other species such as human or Guinea pig. For improved specificity and lower background, or in multiplexed applications, consider using highly cross-adsorbed PhenoVue Fluor conjugated secondary antibodies as an alternative.
Additional product information
Features
| Numbers of vials per unit | 1 |
|---|---|
| Quantity or volume per unit | 1 mg |
| Form | Lyophilized |
| Storage | 2-8°C |
| Recommended working concentration | 1.5 µg/mL (10nM) |
| Maximum excitation wavelength | 650 nm |
| Maximum emission wavelength | 670 nm |
| Common filter set | Cy5 |
| Live cell staining | No |
| Fixed cell staining | Yes |
| Equivalent number of microplates | 25-70 x 96-well microplates 20-70 x 384-well microplates 35-90 x 1536-well microplates |
Specifications
| Color |
Red
|
|---|
| Brand |
PhenoVue™
|
|---|---|
| Detection Method |
Fluorescence
|
| Filter |
Cy5
|
| Fluorophore |
PhenoVue™ Fluor 647
|
| Host |
Donkey
|
| Product Compatibility |
Fixed samples only
|
| Target Species |
Rabbit
|
| Type |
Individual Reagent
|
| Unit Size |
1 vial
|
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Resources
SDS, COAs, Manuals and more
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