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PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed

PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green) + PhenoVue Hoechst 33342 (nucleus, blue) (in PBS). Imaged on Operetta CLS. View All
PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green) + PhenoVue Hoechst 33342 (nucleus, blue) (in PBS). Imaged on Operetta CLS.
PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green) + PhenoVue Hoechst 33342 (nucleus, blue) (in PBS). Imaged on Operetta CLS.
PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green) + PhenoVue Hoechst 33342 (nucleus, blue) (in PBS). Imaged on Operetta CLS.
PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green) + PhenoVue Hoechst 33342 (nucleus, blue) (in PBS). Imaged on Operetta CLS.

Highly cross-adsorbed goat anti-mouse IgG (H+L) antibody conjugated with PhenoVue™ Fluor 488.

PhenoVue Fluor 488 secondary antibody conjugates exhibit bright green fluorescence and are validated for use in high-content screening applications.

Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue Fluor - 488 goat anti-mouse antibody, highly cross-adsorbed, has a maximum excitation wavelength of 495nm and a maximum emission wavelength of 520nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information
Feature Specification
Color Green
Filter FITC
Fluorophore PhenoVue™ Fluor 488
Host Species Goat
Target Species Mouse
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Highly cross-adsorbed goat anti-mouse IgG (H+L) antibody conjugated with PhenoVue™ Fluor 488.

PhenoVue Fluor 488 secondary antibody conjugates exhibit bright green fluorescence and are validated for use in high-content screening applications.

Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue Fluor - 488 goat anti-mouse antibody, highly cross-adsorbed, has a maximum excitation wavelength of 495nm and a maximum emission wavelength of 520nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information
Product Variant
Quantity: 1 x 1 mg
Part #:
2GXM488H1
List Price
USD 258.44
Your online price:
For research use only. Not for use in diagnostic procedures.
Total list price:
USD 258.44
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PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green) + PhenoVue Hoechst 33342 (nucleus, blue) (in PBS). Imaged on Operetta CLS.
PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green) + PhenoVue Hoechst 33342 (nucleus, blue) (in PBS). Imaged on Operetta CLS.
PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green) + PhenoVue Hoechst 33342 (nucleus, blue) (in PBS). Imaged on Operetta CLS.
PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green) + PhenoVue Hoechst 33342 (nucleus, blue) (in PBS). Imaged on Operetta CLS.
PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green) + PhenoVue Hoechst 33342 (nucleus, blue) (in PBS). Imaged on Operetta CLS.
PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green) + PhenoVue Hoechst 33342 (nucleus, blue) (in PBS). Imaged on Operetta CLS.

Product information

Overview

PhenoVue Fluor 488 - goat anti-mouse IgG (H+L) antibody, highly cross-adsorbed, has been cross-adsorbed against various IgG species such as rat, hamster, human, rabbit bovine, horse, or chicken serum to minimize cross-reactivity with other species.

Specificity and sensitivity are improved and background reduced with PhenoVue Fluor 488 - goat anti-mouse IgG (H+L) antibody, highly cross-adsorbed.

It can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications. ​​

Additional product information

Features
Numbers of vials per unit 1
Quantity or volume per unit 1 mg
Form Lyophilized
Storage 2-8°C
Recommended working concentration 1.5 µg/mL (10nM)
Maximum excitation wavelength 495 nm
Maximum emission wavelength 520 nm
Common filter set FITC
Live cell staining No
Fixed cell staining Yes
Equivalent number of microplates 25-70 x 96-well microplates
20-70 x 384-well microplates
35-90 x 1536-well microplates

 

Specifications

Color
Green
Form
Lyophilized
Maximum Emission Wavelength (Emmax)
520 nm
Maximum Excitation Wavelength (Exmax)
495 nm
Application
High Content Imaging
Microscopy
Brand
PhenoVue™
Cross-Adsorption
Highly cross-adsorbed
Detection Modality
Fluorescence
Filter
FITC
Fluorophore
PhenoVue™ Fluor 488
Host Species
Goat
Quantity
1 x 1 mg
Sample Type
Fixed samples only
Shipping Conditions
Shipped Ambient
Storage Conditions
2-8 °C, protected from light
Target Species
Mouse
Type
Individual Reagent

Image gallery

PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green) + PhenoVue Hoechst 33342 (nucleus, blue) (in PBS). Imaged on Operetta CLS.
PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green) + PhenoVue Hoechst 33342 (nucleus, blue) (in PBS). Imaged on Operetta CLS.
PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green) + PhenoVue Hoechst 33342 (nucleus, blue) (in PBS). Imaged on Operetta CLS.
PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green) + PhenoVue Hoechst 33342 (nucleus, blue) (in PBS). Imaged on Operetta CLS.
PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green) + PhenoVue Hoechst 33342 (nucleus, blue) (in PBS). Imaged on Operetta CLS.
PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 488 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, green) + PhenoVue Hoechst 33342 (nucleus, blue) (in PBS). Imaged on Operetta CLS.

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PhenoVue Cellular Imaging Reagents Brochure
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PhenoVue Cellular Imaging Reagents Flyer

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PhenoVue Fluor - Goat Anti-Mouse Secondary Antibodies Product Information Sheet

This is a product information sheet for PhenoVue Fluor - Goat anti-Mouse Secondary Antibodies.

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PhenoVue reagents & imaging microplates product list
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