Highly cross-adsorbed donkey anti-mouse IgG (H+L) antibody conjugated with PhenoVue™ Fluor 488.
PhenoVue Fluor 488 secondary antibody conjugates exhibit bright green fluorescence and are validated for use in imaging microscopy and high-content screening applications.
Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue Fluor - 488 donkey anti-mouse antibody, highly cross-adsorbed, has a maximum excitation wavelength of 495nm and a maximum emission wavelength of 520nm.
View our extensive validation data in the Product Information Sheet within the Resources tab below.
For research use only, not for use in diagnostic procedures.
PhenoVue Fluor 488 - donkey anti-mouse, IgG (H+L) antibody, highly cross-adsorbed, has been cross-adsorbed against various IgG species such as rat, mouse, hamster, human, bovine, horse, or chicken serum to minimize cross-reactivity with other species. Specificity and sensitivity are improved and background reduced with PhenoVue Fluor 488 - donkey anti-mouse IgG (H+L) antibody, highly cross-adsorbed.
It can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
Numbers of vials per unit | 1 |
---|---|
Quantity or volume per unit | 1 mg |
Form | Lyophilized |
Storage | 2-8°C |
Recommended working concentration | 1.5 µg/mL (10nM) |
Maximum excitation wavelength | 495 nm |
Maximum emission wavelength | 520 nm |
Common filter set | FITC |
Live cell staining | No |
Fixed cell staining | Yes |
Equivalent number of microplates | 25-70 x 96-well microplates 20-70 x 384-well microplates 35-90 x 1536-well microplates |
Color |
Green
|
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Brand |
PhenoVue™
|
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Detection Method |
Fluorescence
|
Filter |
FITC
|
Fluorophore |
PhenoVue™ Fluor 488
|
Host |
Donkey
|
Product Compatibility |
Fixed samples only
|
Shipping Conditions |
Shipped Ambient
|
Target Species |
Mouse
|
Type |
Individual Reagent
|
Unit Size |
1 vial
|
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