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PhenoVue Fluor 400LS - WGA

PhenoVue Fluor 400LS - WGA
HeLa cells stained with PhenoVue Fluor 400LS - WGA (Golgi & plasma membranes). Imaged on Operetta CLS system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 400LS - WGA, Fluor 488 - Concanavalin A, and Fluor 568 - Phalloidin). Imaged on Operetta CLS system. View All
PhenoVue Fluor 400LS - WGA
PhenoVue Fluor 400LS - WGA
HeLa cells stained with PhenoVue Fluor 400LS - WGA (Golgi & plasma membranes). Imaged on Operetta CLS system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 400LS - WGA, Fluor 488 - Concanavalin A, and Fluor 568 - Phalloidin). Imaged on Operetta CLS system.
PhenoVue Fluor 400LS - WGA
HeLa cells stained with PhenoVue Fluor 400LS - WGA (Golgi & plasma membranes). Imaged on Operetta CLS system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 400LS - WGA, Fluor 488 - Concanavalin A, and Fluor 568 - Phalloidin). Imaged on Operetta CLS system.
PhenoVue Fluor 400LS - WGA
PhenoVue Fluor 400LS - WGA
HeLa cells stained with PhenoVue Fluor 400LS - WGA (Golgi & plasma membranes). Imaged on Operetta CLS system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 400LS - WGA, Fluor 488 - Concanavalin A, and Fluor 568 - Phalloidin). Imaged on Operetta CLS system.
PhenoVue Fluor 400LS - WGA
HeLa cells stained with PhenoVue Fluor 400LS - WGA (Golgi & plasma membranes). Imaged on Operetta CLS system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 400LS - WGA, Fluor 488 - Concanavalin A, and Fluor 568 - Phalloidin). Imaged on Operetta CLS system.

PhenoVue Fluor 400LS - WGA is a long Stokes shift fluorescent dye conjugated lectin which displays high affinity for sialic acid and N-acetylglucosamine residues of glycoproteins and glycolipids present at the cellular plasma membranes. It can be used for cellular membrane staining, particularly the Golgi apparatus.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue Fluor 400LS - WGA exhibits bright orange fluorescence and can be easily multiplexed for imaging and high-content screening applications. It has been validated on Revvity's HCS instruments such as Operetta CLS and Opera Phenix Plus, and has a maximum excitation wavelength of 395 nm and a maximum emission wavelength of 585 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below. 

For research use only. Not for use in diagnostic procedures.

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Feature Specification
Color Orange
Filter Ex: 375-440 nm, Em: 550-650 nm
Fluorophore PhenoVue™ Fluor 400LS
Organelle and Cell Compartment Golgi Apparatus
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PhenoVue Fluor 400LS - WGA is a long Stokes shift fluorescent dye conjugated lectin which displays high affinity for sialic acid and N-acetylglucosamine residues of glycoproteins and glycolipids present at the cellular plasma membranes. It can be used for cellular membrane staining, particularly the Golgi apparatus.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue Fluor 400LS - WGA exhibits bright orange fluorescence and can be easily multiplexed for imaging and high-content screening applications. It has been validated on Revvity's HCS instruments such as Operetta CLS and Opera Phenix Plus, and has a maximum excitation wavelength of 395 nm and a maximum emission wavelength of 585 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below. 

For research use only. Not for use in diagnostic procedures.

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
View product information
Product Variant
Quantity: 2 x 1 mg
Part #:
CP14001
List Price
USD 393.00
PhenoVue Fluor 400LS - WGA
HeLa cells stained with PhenoVue Fluor 400LS - WGA (Golgi & plasma membranes). Imaged on Operetta CLS system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 400LS - WGA, Fluor 488 - Concanavalin A, and Fluor 568 - Phalloidin). Imaged on Operetta CLS system.
PhenoVue Fluor 400LS - WGA
HeLa cells stained with PhenoVue Fluor 400LS - WGA (Golgi & plasma membranes). Imaged on Operetta CLS system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 400LS - WGA, Fluor 488 - Concanavalin A, and Fluor 568 - Phalloidin). Imaged on Operetta CLS system.
PhenoVue Fluor 400LS - WGA
PhenoVue Fluor 400LS - WGA
HeLa cells stained with PhenoVue Fluor 400LS - WGA (Golgi & plasma membranes). Imaged on Operetta CLS system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 400LS - WGA, Fluor 488 - Concanavalin A, and Fluor 568 - Phalloidin). Imaged on Operetta CLS system.
PhenoVue Fluor 400LS - WGA
HeLa cells stained with PhenoVue Fluor 400LS - WGA (Golgi & plasma membranes). Imaged on Operetta CLS system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 400LS - WGA, Fluor 488 - Concanavalin A, and Fluor 568 - Phalloidin). Imaged on Operetta CLS system.
PhenoVue Fluor 400LS - WGA
PhenoVue Fluor 400LS - WGA
PhenoVue Fluor 400LS - WGA
HeLa cells stained with PhenoVue Fluor 400LS - WGA (Golgi & plasma membranes). Imaged on Operetta CLS system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 400LS - WGA, Fluor 488 - Concanavalin A, and Fluor 568 - Phalloidin). Imaged on Operetta CLS system.
PhenoVue Fluor 400LS - WGA
HeLa cells stained with PhenoVue Fluor 400LS - WGA (Golgi & plasma membranes). Imaged on Operetta CLS system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 400LS - WGA, Fluor 488 - Concanavalin A, and Fluor 568 - Phalloidin). Imaged on Operetta CLS system.

Product information

Overview

WGA is a plant lectin that binds different carbohydrate motifs and displays high affinity for sialic acid and N-acetylglucosamine residues of glycoproteins and glycolipids present at the cellular plasma membranes.

Fluorescent WGA derivatives are commonly used for staining the cellular membranes of mammalian cells, particularly Golgi apparatus which is glycoprotein-enriched. In addition, fluorescent WGA derivatives are also routinely used for the staining of skeletal muscle cells and tissues, as well as in cardiac fibrosis.

PhenoVue Fluor 400LS - WGA can be used to visualize cellular membranes in immunofluorescence as well as high-content analysis and screening applications. ​​
​​Its unique photophysical property enables extended multiplexing capability by adding a fifth color while ensuring no spectral overlap, high brightness as well as photostability.

Features:

  • Number of Vials Per Unit : 2
  • Quantity or Volume Per Vial: 1mg (29.2 nmoles)
  • Form: Lyophilized
  • Storage: 2-8 °C
  • Recommended Working Concentration: 25 µg/mL
  • Maximum Excitation Wavelength: 395nm
  • Maximum Emission Wavelength: 585nm
  • Live Cell Staining: Yes
  • Fixed Cell Staining: Yes
  • Equivalent Number of Microplates:
    • 2 - 8 x 96-well microplates
    • 2 - 8 x 384-well microplates
    • 4 - 13 x 1536-well microplates

Specifications

Color
Orange
Form
Lyophilized
Maximum Emission Wavelength (Emmax)
585 nm
Maximum Excitation Wavelength (Exmax)
395 nm
Application
High Content Imaging
Microscopy
Brand
PhenoVue™
Detection Modality
Fluorescence
Filter
Ex: 375-440 nm, Em: 550-650 nm
Fluorophore
PhenoVue™ Fluor 400LS
Organelle and Cell Compartment
Golgi Apparatus
Quantity
2 x 1 mg
Sample Type
Live and fixed samples
Shipping Conditions
Shipped Ambient
Storage Conditions
2-8 °C, protected from light
Type
Individual Reagent

Image gallery

PhenoVue Fluor 400LS - WGA
HeLa cells stained with PhenoVue Fluor 400LS - WGA (Golgi & plasma membranes). Imaged on Operetta CLS system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 400LS - WGA, Fluor 488 - Concanavalin A, and Fluor 568 - Phalloidin). Imaged on Operetta CLS system.
PhenoVue Fluor 400LS - WGA
HeLa cells stained with PhenoVue Fluor 400LS - WGA (Golgi & plasma membranes). Imaged on Operetta CLS system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 400LS - WGA, Fluor 488 - Concanavalin A, and Fluor 568 - Phalloidin). Imaged on Operetta CLS system.
PhenoVue Fluor 400LS - WGA
PhenoVue Fluor 400LS - WGA
HeLa cells stained with PhenoVue Fluor 400LS - WGA (Golgi & plasma membranes). Imaged on Operetta CLS system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 400LS - WGA, Fluor 488 - Concanavalin A, and Fluor 568 - Phalloidin). Imaged on Operetta CLS system.
PhenoVue Fluor 400LS - WGA
HeLa cells stained with PhenoVue Fluor 400LS - WGA (Golgi & plasma membranes). Imaged on Operetta CLS system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 400LS - WGA, Fluor 488 - Concanavalin A, and Fluor 568 - Phalloidin). Imaged on Operetta CLS system.
PhenoVue Fluor 400LS - WGA
PhenoVue Fluor 400LS - WGA
HeLa cells stained with PhenoVue Fluor 400LS - WGA (Golgi & plasma membranes). Imaged on Operetta CLS system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 400LS - WGA, Fluor 488 - Concanavalin A, and Fluor 568 - Phalloidin). Imaged on Operetta CLS system.
PhenoVue Fluor 400LS - WGA
HeLa cells stained with PhenoVue Fluor 400LS - WGA (Golgi & plasma membranes). Imaged on Operetta CLS system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 400LS - WGA, Fluor 488 - Concanavalin A, and Fluor 568 - Phalloidin). Imaged on Operetta CLS system.

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PhenoVue Cellular Imaging Reagents Brochure
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PhenoVue Cellular Imaging Reagents Flyer

This flyer describes Revvity's PhenoVue cellular imaging reagents.

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PhenoVue Fluor - WGA Conjugates Product Information Sheet

Fluorescent WGA conjugates represent a method of choice for labelling the cellular membranes of mammalian cells, particularly...

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PhenoVue Reagents & Imaging Microplates Product List
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