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PhenoVue 505 Live Cell Caspase-3/7 Activity Stain
PhenoVue 505 Live Cell Caspase-3/7 Activity Stain
PhenoVue™ 505 live cell caspase-3/7 activity stain is a no wash, cell permeable fluorogenic dye which is specifically cleaved by activated pro apoptotic caspases 3 and 7 in living cells, leading to fluorescence increase.
Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue 505 live cell caspase-3/7 activity stain, has a maximum excitation wavelength of 505 nm and a maximum emission wavelength of 530 nm.
View our extensive validation data in the Product Information Sheet within the Resources tab below.
For research use only. Not for use in diagnostic procedures.
| Feature | Specification |
|---|---|
| Cell Function Indicator | Apoptosis indicator |
| Color | Green |
| Filter | FITC |
PhenoVue™ 505 live cell caspase-3/7 activity stain is a no wash, cell permeable fluorogenic dye which is specifically cleaved by activated pro apoptotic caspases 3 and 7 in living cells, leading to fluorescence increase.
Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue 505 live cell caspase-3/7 activity stain, has a maximum excitation wavelength of 505 nm and a maximum emission wavelength of 530 nm.
View our extensive validation data in the Product Information Sheet within the Resources tab below.
For research use only. Not for use in diagnostic procedures.
PhenoVue 505 Live Cell Caspase-3/7 Activity Stain
PhenoVue 505 Live Cell Caspase-3/7 Activity Stain
Product information
Overview
PhenoVue 505 live cell caspase-3/7 activity stain is a no wash, cell permeable fluorogenic dye which is specifically cleaved by activated pro apoptotic caspases 3 and 7 in living cells, leading to fluorescence increase.
Sensitive, rapid and photostable, PhenoVue 505 live cell caspase-3/7 activity stain exhibits green emission and can be multiplexed with blue, orange and red colors.
PhenoVue 505 live cell caspase-3/7 activity stain uses a DEVD substrate conjugated with a non-fluorescent cell permeable DNA binding dye which becomes fluorescent when cells undergo apoptosis.
Additional product information
Features
| Numbers of vials per unit | 1 |
|---|---|
| Quantity or volume per unit | 100 µL (1 mM DMSO) |
| Form | Liquid |
| Storage | -16 °C |
| Recommended working concentration | 5 µM |
| Maximum excitation wavelength | 505 nm |
| Maximum emission wavelength | 530 nm |
| Common filter set | FITC |
| Live cell staining | Yes |
| Fixed cell staining | No |
| Equivalent number of microplates | 0.5-2 x 96-well microplates 0.5-2 x 384-well microplates 1-3 x 1536-well microplates |
Specifications
| Color |
Green
|
|---|---|
| Form |
Solution in DMSO
|
| Maximum Emission Wavelength (Emmax) |
530 nm
|
| Maximum Excitation Wavelength (Exmax) |
505 nm
|
| Application |
High Content Imaging
Microscopy
|
|---|---|
| Brand |
PhenoVue™
|
| Cell Function Indicator |
Apoptosis indicator
|
| Detection Modality |
Fluorescence
|
| Filter |
FITC
|
| Quantity |
1 x 100 µL
|
| Sample Type |
Live samples only
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Storage Conditions |
-16 °C or below, protected from light
|
| Type |
Individual Reagent
|
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Resources
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This flyer describes Revvity's PhenoVue cellular imaging reagents.
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