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NEXTFLEX Universal Blockers

The NEXTFLEX® Universal Blockers are short DNA oligos that block non-specific hybridization between adapter sequences, enhancing specificity and reducing sequencing costs. They are compatible with all NEXTFLEX barcodes and all other ligation and tagmentation based workflows for Illumina® and Element® sequencing platforms. Also known as adapter-blocking oligos or universal U-Block mixes, Universal Blockers prevent index-to-index cross-hybridization in TruSeq®, Nextera™ and Element® libraries. 

Feature Specification
Automation Compatible No
Product Group Targeted Sequencing

The NEXTFLEX® Universal Blockers are short DNA oligos that block non-specific hybridization between adapter sequences, enhancing specificity and reducing sequencing costs. They are compatible with all NEXTFLEX barcodes and all other ligation and tagmentation based workflows for Illumina® and Element® sequencing platforms. Also known as adapter-blocking oligos or universal U-Block mixes, Universal Blockers prevent index-to-index cross-hybridization in TruSeq®, Nextera™ and Element® libraries. 

Product variants
Unit Size: 8 rxns
Part #:
NOVA-5143231
List price
USD 113.41
Your online price:
Unit Size: 48 rxns
Part #:
NOVA-5143232
List price
USD 237.54
Your online price:
Unit Size: 96 rxns
Part #:
NOVA-5143233
List price
USD 373.42
Your online price:
For research use only. Not for use in diagnostic procedures.
Total list price:
USD 237.54
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Overview

Simplify and reduce the cost of hybridization-based target capture with the NEXTFLEX universal blockers for NGS
  • Effective in blocking without being affected by index length or the presence of a unique molecular index (UMI)
  • Compatible with all NEXTFLEX barcodes and all other ligation and tagmentation based workflows for Illumina and Element sequencing platforms
  • Simplifies and reduces the cost of target capture without sacrificing performance.

Additional product information

Improve the number of on-target reads


How adapter-blocking oligos increase on-target reads

During target enrichment, adapters can interact with the complementary adapter sequence strand of another library molecule. The NEXTFLEX Universal Blockers are designed to prevent this concatemerization of library molecules. By blocking this complementary adapter sequence interaction, the number of on-target reads improves dramatically and the depth of enrichment increases.

One set of blockers for all applications

The NEXTFLEX Universal Blockers are designed for use with indexed adapters for Illumina sequencing platforms. They are effective in blocking single-indexed or dual-indexed libraries, without being affected by index length or the presence of a unique molecular index (UMI).

nextflex universal blockers figure 1


Figure 1. NEXTFLEX Universal Blockers (UB) work from 0-19 bp unblocked regions (barcodes and/or UMIs). Competitor A1- and A-3 are two separate products from Competitor A.

nextflex universal blockers figure 2


Figure 2. NEXTFLEX Universal Blockers can block Illumina TruSeq™ or Nextera™ -style libraries. Competitor A1- and A-2 are two separate products from Competitor A.

The NEXTFLEX Universal Blockers are compatible with ligation-based workflows, such as NEXTFLEX or Illumina TruSeq library prep kits, and with any libraries prepared with NEXTFLEX Dual-Index Barcodes or NEXTFLEX Unique Dual Index Barcodes.

They are also compatible with tagmentation-based workflows, such as Illumina Nextera library prep kits.

Specifications

Automation Compatible
No
Product Group
Targeted Sequencing
Shipping Conditions
Shipped in Dry Ice
Unit Size
48 rxns

Citations

  • Tian, Yijun, et al. "Identify Regulatory eQTLs by Multiome Sequencing in Prostate Single Cells." bioRxiv, 21 June 2024, doi:10.1101/2024.06.19.599704. https://pmc.ncbi.nlm.nih.gov/articles/PMC11213234/

FAQs

  • What are universal blockers?
  • Do universal blockers work with 6 bp and 10 bp dual indexes?
  • Can I use universal blockers with Twist or IDT libraries?
  • What concentration should I use the NEXTFLEX Universal blockers at?
  • How should I store the universal blockers?

Resources

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Simply & reduce the cost of hybridization-based target capture with universal blockers

This flyer describes the benefits of the NEXTFLEX® Universal Blockers

SDS, COAs, manuals and more

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Safety data sheet
IFUs, manuals & more
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Adapters (10NT index)

NEXTFLEX NGS Library Prep
Part number:NOVA-534100
List price:USD 20,655.00/each
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NEXTFLEX NGS Library Prep
Part number:NOVA-534101
List price:USD 5,164.26/each
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USD 0
NEXTFLEX NGS Library Prep
Part number:NOVA-534102
List price:USD 5,164.26/each
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USD 0
NEXTFLEX NGS Library Prep
Part number:NOVA-534103
List price:USD 5,164.26/each
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USD 0
NEXTFLEX NGS Library Prep
Part number:NOVA-534104
List price:USD 5,164.26/each
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USD 0

Adapters (8NT index)

NEXTFLEX NGS Library Prep
Part number:NOVA-514150
List price:USD 1,958.40/each
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USD 0
NEXTFLEX NGS Library Prep
Part number:NOVA-514150-EVAL16
List price:USD 163.20/each
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USD 0
NEXTFLEX NGS Library Prep
Part number:NOVA-514150-EVAL48
List price:USD 489.60/each
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USD 0

Cell-Free DNA-seq Library Prep

NEXTFLEX NGS Library Prep
Part number:NOVA-5150-01
List price:USD 192.00/each
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USD 0
NEXTFLEX NGS Library Prep
Part number:NOVA-5150-02
List price:USD 1,152.00/each
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USD 0

DNA-seq Library Prep (Enzymatic Fragmentation)

NEXTFLEX NGS Library Prep
Part number:NOVA-5149-21
List price:USD 192.00/each
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USD 0
NEXTFLEX NGS Library Prep
Part number:NOVA-5149-22
List price:USD 1,152.00/each
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USD 0
NEXTFLEX NGS Library Prep
Part number:NOVA-5149-23
List price:USD 2,304.00/each
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USD 0

DNA-seq Library Prep (Mechanical Fragmentation)

NEXTFLEX NGS Library Prep
Part number:NOVA-5188-01
List price:USD 192.00/each
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USD 0
NEXTFLEX NGS Library Prep
Part number:NOVA-5188-02
List price:USD 1,152.00/each
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USD 0
NEXTFLEX NGS Library Prep
Part number:NOVA-5188-03
List price:USD 2,304.00/each
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