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NEXTFLEX Rapid DNA-Seq Kit 2.0
The NEXTFLEX® Rapid DNA-seq 2.0 kit is designed to prepare multiplexed libraries for single or paired-end sequencing using Illumina® and Element Biosciences® platforms from fragmented DNA. This kit does not include enzymatic fragmentation.
For research use only. Not for use in diagnostic procedures.
Product Variants
Product information
Overview
- Complete library prep solution, including size selection beads
- Up to 1,536 UDI barcodes are available separately
- Convenient and cost-effective package in an 8, 48, or 96 reaction format
- Simplified workflow
- Robust genome coverage
- Reliable performance with GC-bias and sequencing bias mitigation
- Low input requirement for genomic DNA down to 1 ng
- Functionally tested with Illumina® sequencing platform
- Automation compatible with liquid handlers, including Revvity’s Sciclone® G3 NGSx and Zephyr® G3 NGS workstations
Additional product information
The NEXTFLEX Rapid DNA-seq kit 2.0 produces library preps with a larger number of unique sequencing reads, allowing for robust genome coverage in challenging GC-rich sequences that can introduce bias.
Figure 1: Libraries prepared with NEXTFLEX rapid DNA-Seq kit 2.0 show higher yield than libraries prepared with Competitor K’s kit using the same number of PCR cycles from a 250 ng and 1 ng input (A) and robust conversion rate (B).
Additional Data between NEXTFLEX Rapid DNA-Seq 2.0 and Competitor K’s Kit Libraries
Figure 2: Libraries prepared with NEXTFLEX Rapid DNA-seq 2.0 shows less GC bias than libraries prepared with Competitor K’s kit.
Figure 3: Libraries prepared with NEXTFLEX Rapid DNA-seq 2.0 shows higher genome coverage than libraries prepared with Competitor K’s kit.
Flexible Multiplexing Options
The NEXTFLEX adapters are long, annealed adapters containing indexed sequences, which offer an improved multiplexing workflow and flexible experimental setups catered to individual needs. Our barcode portfolio encompasses options for single or paired-end multiplex sequencing. The NEXTFLEX Rapid DNA-seq kit 2.0 reagents are compatible with the NEXTFLEX Unique Dual Index Barcodes for up to 1,536 sample multiplexing.
Automation Compatibility
The NEXTFLEX Rapid DNA-seq Kit 2.0 is compatible with multiple liquid handlers, including the Revvity Sciclone G3 NGSx and Zephyr G3 NGS workstations.
Specifications
| Barcodes |
No Barcodes Provided
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|---|---|
| Brand |
NEXTFLEX
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| Shipping Conditions |
Dual Temperature
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| Type |
DNA-seq
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| Unit Size |
8 rxns
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Resources
Application Note
NEXTFLEX Rapid 2.0 DNA-Seq App Note
This application note describes how the NEXTFLEX® rapid DNA-seq kit 2.0 improves the efficiency of each step to generate high...
SDS, COAs, Manuals and more
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