NEXTFLEX 16S V4 Amplicon-Seq Kit 2.0

Low Input Requirements for 16S rRNA Sequencing

As little as 1 ng of genomic DNA can be used to generate libraries using the NEXTFLEX 16S V4 Amplicon-Seq Library Prep Kit 2.0.

Fig 1. Genera measured by analysis of 16S V4 reads

Optimized Protocol Offers Lower PCR Bias and Fewer Off-target Reads

The NEXTFLEX 16S V4 Amplicon-Seq Kit 2.0 incorporates a second PCR step in the protocol for the addition of the sample-specific index which reduces off-target reads encountered during amplicon sequencing.

384 Barcodes Available for Cost-effective 16S V4 rRNA Sequencing

The NEXTFLEX 16S V4 Amplicon-Seq Library Prep Kit 2.0 can be used for multiplexing up to 384 samples, to greatly reduce the cost of sequencing. The barcodes are offered in sets of 12, 48 or 96 barcodes and supplied with 2 reactions worth of each barcode.

Automate your 16S V4 Library Prep to Increase Throughput and Reduce Errors

A NEXTFLEX^® automation protocol is now available on the Sciclone^® NGS and NGSx workstations to help labs increase their throughput and reduce human errors.

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