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  • Home
  • Products
  • Research & Development
  • Genomic Analysis
  • Library Prep Kits
  • NEXTFLEX 16S V4 Amplicon-Seq Kit 2.0

NEXTFLEX 16S V4 Amplicon-Seq Kit 2.0

NEXTFLEX NGS Library Prep
NEXTFLEX 16S V4 Amplicon-Seq Kit 2.0
NEXTFLEX NGS Library Prep
Revvity kit box for reagents and consumables
NEXTFLEX NGS Library Prep

The NEXTFLEX® 16S V4 Amplicon-Seq Library Prep Kit 2.0 is designed for the preparation of multiplexed amplicon libraries spanning the V4 hypervariable domain of microbial 16S ribosomal RNA (rRNA) genes. These libraries are compatible with paired-end sequencing on the Illumina® and Element Biosciences® sequencing platforms.

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Feature Specification
Automation Compatible Yes
Product Group 16S amplicon-seq
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The NEXTFLEX® 16S V4 Amplicon-Seq Library Prep Kit 2.0 is designed for the preparation of multiplexed amplicon libraries spanning the V4 hypervariable domain of microbial 16S ribosomal RNA (rRNA) genes. These libraries are compatible with paired-end sequencing on the Illumina® and Element Biosciences® sequencing platforms.

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NEXTFLEX NGS Library Prep
NEXTFLEX 16S V4 Amplicon-Seq Kit 2.0
NEXTFLEX NGS Library Prep
Revvity kit box for reagents and consumables
NEXTFLEX 16S V4 Amplicon-Seq Kit 2.0
NEXTFLEX NGS Library Prep
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  • SDS, COAs, manuals and more
  • Related products
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Product information

  • Overview
  • Specifications
  • References

Overview

16S V4 rRNA library prep for broad-range analysis of mixed microbial populations

  • Fast and easy library prep protocol
  • Low input – Only 1 ng of input required
  • Multiplex up to 384 libraries
  • Custom sequencing primers are not required
  • Automation protocols are now available for the Sciclone® NGS and NGSx Workstations to automate your 16S sequencing
  • Functionally tested with Illumina® sequencing platforms

Analysis powered by Cosmos-Hub®

The NEXTFLEX 16S V4 rRNA Amplicon-seq panels are now available bundled with access to Cosmos-Hub; an online software solution that enables fast and easy analysis of complex microbiome data. Cosmos-Hub gives scientists user-friendly access to version-controlled and validated 16S pipelines. The machine-learning powered software also enables rapid data interpretation through a comparative analysis software with features such as tables, heatmaps, bar charts, multiple Alpha & Beta Diversity indexes, abundance distribution plots, differential abundance testing, as well as comprehensive statistics between groups. Learn more about Cosmos-Hub.

Revvity 16S rRNA analysis - online demonstration

Now you can schedule a one-on-one 30-minute online demonstration that will cover everything you need to know about the Cosmos-Hub® which is now available with Revvity’s NEXTFLEX™ 16S rRNA Amplicon-seq Kits. You will be able to see the analysis demonstrated and our experts will be able to answer any questions that you may have. Please choose a timeframe that suits you best and we will provide you a Microsoft Teams personal link to join when the time comes. We look forward to speaking with you.

Specifications

Analysis
Included
Automation Compatible
Yes
Barcodes
1 - 12
Product Group
16S amplicon-seq
Shipping Conditions
Shipped in Dry Ice
Unit Size
24 rxns

References

  • Paruch L et al. 2020. Seasonal dynamics of lotic bacterial communities assessed by 16S rRNA gene amplicon deep sequencing. Scientific Reports 10:16399.
  • Curry KD et al. 2022. Emu: Species-Level Microbial Community Profiling for Full-Length Nanopore 16S Reads. Nature Methods 19(7):845–853.
  • Argaw-Denboba A et al. 2024. Paternal microbiome perturbations impact offspring fitness. Nature 629:652–659.

Resources

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1-1 of 1 Resources
Application Note Icon
Application Note
Automated homogenization and DNA extraction from murine GI tract tissue, cultures, and mock community for 16S sequencing

The success of sensitive downstream genomic applications such as qPCR depend on robust and reliable upstream sample preparation...

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