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  • Library Prep Kits
  • NEXTFLEX 16S V1-V3 rRNA Amplicon-seq Kits, 48 Barcodes

NEXTFLEX 16S V1-V3 rRNA Amplicon-seq Kits, 48 Barcodes

NEXTFLEX NGS Library Prep
NEXTFLEX 16S V1-V3 rRNA Amplicon-seq Kits, 48 Barcodes
NEXTFLEX NGS Library Prep
Revvity kit box for reagents and consumables
NEXTFLEX NGS Library Prep

Library preparation kits for 16S V1-V3 rRNA bacterial metagenomics analysis for Illumina® and Element Biosciences® NGS platforms.

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Feature Specification
Automation Compatible Yes
Product Group 16S amplicon-seq
Request more information

Library preparation kits for 16S V1-V3 rRNA bacterial metagenomics analysis for Illumina® and Element Biosciences® NGS platforms.

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Product variants
Unit Size: 24 rxns
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Barcodes
1 - 12
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NOVA-4202-02S
List price
USD 806.82
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Unit Size: 24 rxns
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NOVA-4202-02
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USD 562.02
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Unit Size: 96 rxns
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USD 3,102.84
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Unit Size: 96 rxns
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NOVA-4202-03
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USD 2,124.66
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Unit Size: 192 rxns
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USD 5,989.44
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Unit Size: 192 rxns
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NOVA-4202-04
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USD 4,031.04
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Unit Size: 192 rxns
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Unit Size: 192 rxns
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97 - 192
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NOVA-4202-05
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Unit Size: 192 rxns
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NOVA-4202-06S
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Unit Size: 192 rxns
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NOVA-4202-06
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Unit Size: 192 rxns
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Part #:
NOVA-4202-07S
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For research use only. Not for use in diagnostic procedures.
Total list price:
USD 2,124.66
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NEXTFLEX NGS Library Prep
NEXTFLEX 16S V1-V3 rRNA Amplicon-seq Kits, 48 Barcodes
NEXTFLEX NGS Library Prep
Revvity kit box for reagents and consumables
NEXTFLEX 16S V1-V3 rRNA Amplicon-seq Kits, 48 Barcodes
NEXTFLEX NGS Library Prep
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  • SDS, COAs, manuals and more
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Product information

  • Overview
  • Specifications
  • Citations

Overview

The NEXTFLEX® 16S V1 - V3 rRNA Amplicon-Seq Library Prep Kit is designed for the preparation of multiplexed amplicon libraries that span the hypervariable domains one through three (V1-V3) of microbial 16S ribosomal RNA (rRNA) genes. These libraries are compatible with paired-end sequencing on the Illumina® sequencing platforms.

  • Up to 384 barcodes for multiplexing
  • Practical kit sizes for low and high‑throughput projects
  • Streamlined workflow
  • Automated on Revvity’s suite of liquid handlers to increase your throughput

By utilizing NGS, entire bacterial communities within a sample can be efficiently analyzed from minimal quantities of DNA, without biases that could potentially be introduced by culturing in other non-NGS methods. The NEXTFLEX 16S rRNA amplicon-seq kits maximize the economy and speed of targeted sequencing by offering exceptional values for labs needing a metagenomics solution.

Analysis powered by Cosmos-Hub®

The NEXTFLEX 16S V1 - V3 rRNA Amplicon-seq panels are now available bundled with access to Cosmos-Hub; an online software solution that enables fast and easy analysis of complex microbiome data. Cosmos-Hub gives scientists user-friendly access to version-controlled and validated 16S pipelines. The machine-learning powered software also enables rapid data interpretation through a comparative analysis software with features such as tables, heatmaps, bar charts, multiple Alpha & Beta Diversity indexes, abundance distribution plots, differential abundance testing, as well as comprehensive statistics between groups. Learn more about Cosmos-Hub.

Revvity 16S rRNA analysis - online demonstration

Now you can schedule a one-on-one 30-minute online demonstration that will cover everything you need to know about the Cosmos-Hub® which is now available with Revvity’s NEXTFLEX™ 16S rRNA Amplicon-seq Kits. You will be able to see the analysis demonstrated and our experts will be able to answer any questions that you may have. Please choose a timeframe that suits you best and we will provide you a Microsoft Teams personal link to join when the time comes. We look forward to speaking with you.

Specifications

Analysis
Not Included
Automation Compatible
Yes
Barcodes
1 - 48
Product Group
16S amplicon-seq
Shipping Conditions
Shipped in Dry Ice
Unit Size
96 rxns

Citations

  • L’Heureux, J. E., Corbett, A., Ballard, C., … & Vanhatalo, A. (2025). Oral microbiome and nitric oxide biomarkers in older individuals with APOE4 genotype and mild cognitive impairment. PNAS Nexus, 4(1), pgae543. https://doi.org/10.1093/pnasnexus/pgae543
  • Vanhatalo, A., Curtis, K. J., Littlewood, J., … & James, P. E. (2021). Network analysis of the nitrate-sensitive oral microbiome reveals interactions with cognitive and cardiovascular health. Free Radical Biology and Medicine, 168, 38–52. https://doi.org/10.1016/j.freeradbiomed.2021.02.010
  • Qu, W., Zhou, Y., & Zhang, Y. (2024). Gut microbiota carcinogen metabolism causes distal tissue tumours. Nature, 616, 321–329. https://doi.org/10.1038/s41586-024-07754-w

Resources

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  • Application Note (1)
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1-2 of 2 Resources
Flyer Icon
Flyer
End-to-end metagenomics solutions.

This flyer showcases Revvity's metagenomic portfolio as well as Cosmos-Hub award winning analysis solution.

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Flyer Icon
Flyer
Simplify your 16S library prep and analysis.

This flyer provides guidance on which NEXTFLEX metagenomic kit to use for microbial community profiling projects

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