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  • Who we serve
    • Academia
      Explore BioLegend

      Learn about our world class antibodies for a diverse set of research areas including immunology, neuroscience, cancer, stem cells and cell biology.

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    • Pharma / Biotech
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    • Clinical Laboratories
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    • Healthcare Professionals
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    • Contract Research Organizations
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  • Products
    • Research & Development
      • Genomic Analysis
        • IncRNA in Functional Studies
        • Nucleic Acid Isolation
        • NGS Workflows
        • Microplate Readers
        • Microfluidic Nucleic Acid Analysis
        • CRISPR Technologies
        • RNA Interference
        • Transfection and Ancillary Reagents
        • Oligonucleotide Custom Synthesis
        • cDNAs and ORF Clones
        • Single-Cell Sequencing
        • Labeled Nucleotides
        • qPCR
        • Library Prep Kits
        • Magnetic Beads
        • Viral Vector Products
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      • Protein Analysis
        • Binding Assays
        • Immunoassays
        • Microfluidic Protein Characterization
        • Microplate Readers
        • Microplates
        • Recombinant Proteins
        • Western Blotting
        • Sample Dissociation
        • M-PVA Magnetic Beads
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      • Cell Analysis
        • Cell Isolation
        • Cell Lines & Stem Cells
        • Cell Counting and Image Cytometry
        • Cell Health & Viability
        • Cellular Imaging & Analysis
        • Immunoassays
        • In Vivo Imaging
        • Microplates
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      • Research Solutions
        • Immunoassays Solutions
        • pHSense Reagents
        • Biomarker Discovery
        • Cell and Gene Therapy
        • GMP Workflows
        • Biologics
        • Small Molecule Drug Discovery
        • Disease Research
        • Target Class
        • Drug Development
        • Precision Medicine Research
        • Functional Genomic Screening Solutions
        • Assay Development Workflows
        • Physiological Model Solutions
        • Biobanking Workflows
        • Agrigenomics Workflows
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    • Clinical & Diagnostics
      • Reproductive Health
        • Maternal and Prenatal Testing
        • Newborn Screening
        • Newborn Sequencing Research
        • Pregnancy-Relevant Infections
        • Endocrine Reproductive
        • Cell-free DNA Analysis
        • Neonatal Research
        • Preimplantation Genetic Testing
        • PlGF Testing Research
        • Molecular Cytogenetics
        New IVD Mimix™ reference standards for oncology workflows.

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      • Infectious Diseases
        • Tuberculosis Management
        • EUROIMMUN Solutions
        • IDS Solutions
        • Nucleic Acid Isolation for Pathogen Detection
        • Cytomegalovirus
        • SARS-COV-2 Testing Solutions
        • Bacterial & Viral Nucleic Acid Isolation
        • Metagenomics
        New IVD Mimix™ reference standards for oncology workflows.

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      • Cancer
        • ASR Flow Cytometry Antibodies
        • HPV Testing
        • ctDNA Workflows
        • miRNA-seq Analysis
        • Exosome/cfRNA Analysis
        • Targeted Sequencing
        • Mimix Reference Standards
        • Functional Testing
        NEW! Mimix IVD reference standards

        Providing diagnostic labs with trusted quality controls for developing tests and monitoring workflows, with the added assurance of IVD marking. Explore our newest MimixTM controls.

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      • Autoimmunity
        • EUROIMMUN Solutions
        • IDS Solutions
        New IVD Mimix™ reference standards for oncology workflows.

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      • Endocrinology
        New IVD Mimix™ reference standards for oncology workflows.

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      • Allergy
        New IVD Mimix™ reference standards for oncology workflows.

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      • Neurodegeneration
        • Alzheimer's Disease
        • Amytrophic Lateral Sclerosis (ALS)
        • Neurogranin
        • BACE1
        New IVD Mimix™ reference standards for oncology workflows.

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      • Rapid Patient Testing
        New IVD Mimix™ reference standards for oncology workflows.

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      New IVD Mimix™ reference standards for oncology workflows.

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    • Reagents
      • BioLegend
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      • Cellular Imaging Reagents
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      • chemagic Nucleic Acid Purification Kits
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      • CRISPR Technologies
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      • GPCR Research Reagents
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      • Immunoassays
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      • In Vivo Imaging Reagents
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      • Liquid Scintillation Cocktails
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      • NGS Library Prep Kits
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      • pHSense Reagents
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      • qPCR
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      • Radiochemicals
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      • RNA Interference
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      • Primary and Secondary Antibodies
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    • Platforms & Automation
      • Nucleic Acid Purification
        • chemagic Applications
        • chemagic IVD Instruments
        • chemagic IVD Kits
        • chemagic Instruments
        • chemagic Kits
        • M-PVA Magnetic Beads
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Automated Liquid Handling
        • Liquid Handling Workstations
        • Automated Liquid Handling Applications
        • Liquid Handling Consumables & Accessories
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Integrated Lab Automation
        • Automated Plate Loading
        • Workstation Modules
        • Scheduling & Control Software
        • Robotics
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Microfluidic Analysis
        • Microfluidic Protein Characterization
        • Microfluidic Nucleic Acid Analysis
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Detection Solutions
        • Plate Reader Accessories
        • Microplate Readers
        • Radiometric Detectors
        • Radiochemicals
        • Liquid Scintillation Cocktails
        • Radiometric Consumables & Accessories
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Imaging
        • Cellular Imaging & Analysis
        • In Vivo Imaging
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Cell Counting and Image Cytometry
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Sample Homogenization
        • Homogenizers
        • Homogenizer Accessories
        • Homogenizer Consumables
        • Sample Centrifugation
        • Sample Sonication
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • In Vitro Diagnostics (IVD) Platforms & Automation
        • IVD Nucleic Acid Isolation
        • IVD Automated Liquid Handling
        • IVD Radiometric Detectors
        • EUROIMMUN Instruments
        • IDS Instruments
        • Clinical Applications
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Consumables & Accessories
        • Cassettes
        • Cell Harvesters
        • In Vivo Imaging Accessories
        • High-Content Imaging Accessories
        • Microplates
        • Radioactive Spill Cleaners
        • Scintillation Cocktails
        • Transfer Membranes
        • Charcoal Traps
        • Homogenizer Accessories
        • Homogenizer Consumables
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Instrument Service & Maintenance
        • On Demand Equipment Service
        • Equipment Service Plans
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      New! Cellometer™ Ascend™

      Explore our cell counting and image cytometry solutions.

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    • Consumables & Accessories
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    • Signals Software
      • All Products
        • Signals One
        • ChemDraw
        • Spotfire®
        • Signals Clinical
        • Signals Notebook
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      • All Solutions
        • Biology
        • Discovery Chemistry
        • Clinical & Translational
        • Specialty Chemicals
        • Food/Flavor/Fragrance
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    • Revvity Omics Services
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  • Services
    • Preclinical Services
      • Antibody Drug Conjugate Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Complex Cell Model Screening Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Base Editing Platform
        • Pin-point base editing platform cell line engineering services
        • Pin-point base editing platform pooled tiled screening services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Immune Cell Screening
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Functional Genomic Screening Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Cell Panel Screening
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Cell Line Engineering
        • CHOSOURCE CLE Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Viral Vector Engineering and Manufacture
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      Preclinical services

      Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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    • Revvity Omics Services
      • Revvity Omics Clinical Services
        • Cytogenomics
        • Global Laboratory Network
        • Metabolic Testing
        • Newborn Screening Services
        • Prenatal Screening Services
        • Proactive Testing
        • Rare Disease Testing
        • Specialized and Customized Assays
        • Sponsored Testing Programs
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Revvity Omics Pharma Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Clinical & Testing Services
      • Revvity Omics Clinical Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      • Revvity Omics Pharma Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Cellular and Humoral Immunoassays
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Tuberculosis Testing Services
        • Oxford Diagnostic Laboratories Service Closures
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Customization Services
      • Assays and Reagents
        • Assay Development
        • Compound profiling
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Microplate Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Custom Conjugation & Labeling
        • Custom Labeling
        • Small Molecule
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Radiosynthesis and Labeling
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Licensing
      • Gene Delivery Licensing
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      • Gene Expression Systems
        • CHOSOURCE Platform
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      • Pin-point™ Base Editing Platform
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      • Virus Screening
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      CHOSOURCE expression platform

      Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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    • Viral Vector Engineering and Manufacture
      • AAV Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Lentivirus Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Instrument Service & Maintenance
      • AV Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Equipment Service Plans
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      • On-demand Equipment Service
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

      Learn more

    • Customer Training
      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • OEM Solutions
      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    T-SPOT.TB testing services.

    Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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  • Company
    • Purpose
      • Approach
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      • Our Story
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      • Leadership
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    • Careers
      • Careers Home
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      • Why Revvity
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      • Search Jobs
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    • Investor Relations
      • Events
        Investor Day 2024

        Driving meaningful innovation that profoundly impacts science and human lives.

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      • Financials
        Investor Day 2024

        Driving meaningful innovation that profoundly impacts science and human lives.

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      • Stock Info
        Investor Day 2024

        Driving meaningful innovation that profoundly impacts science and human lives.

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      Investor Day 2024

      Driving meaningful innovation that profoundly impacts science and human lives.

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    • ESG
      • Environmental
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      • Social
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      • Governance
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    • News
      • Announcements
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      • Awards
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      • Media Alerts
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      • Press Coverage
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  • Resources
    • Product Support
      • Application Support Knowledge base (ASK)
        Tech documents, at your fingertips.

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      • SpectraViewer
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      • RAD Calculator
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    • Blog
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    • Events
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    • Customer Training
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    • Help Center
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      • Cell Line Terms & Conditions
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    • Knowledge Base
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      • Sample homogenization applications and protocols
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      • Newborn screening disorders
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      • TB testing services
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      • Investigator led studies
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      • Tuberculosis (TB)
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  • Brands
      Revvity Brands
    • BioLegend

    • EUROIMMUN

    • Revvity Omics

    • Revvity Signals

    • Tulip Diagnostics

    • ViaCord

    • Popular Revvity Product Brands
    • AlphaLISA and SureFire Ultra

    • BioQule NGS

    • chemagic

    • EnVision Nexus

    • Fontus

    • HTRF

    • IVIS

    • LEGENDplex

    • Opera Phenix

    • T-SPOT

    • TotalSeq

    • View all Revvity brands
  • Welcome to Revvity: renowned brands and boundless innovation.

    Hearing the word "can't" is our call to action!

    We help scientists, researchers, and clinicians overcome the world's greatest health obstacles.

    View our story

    Featured brand: BioLegend

    Learn about our world-class antibodies for a diverse set of research areas including immunology, neuroscience, cancer, stem cells and cell biology.

    Visit BioLegend.com

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  • NEXTFLEX 16S V1-V3 rRNA Amplicon-seq Kits, 12 Barcodes

NEXTFLEX 16S V1-V3 rRNA Amplicon-seq Kits, 12 Barcodes

NEXTFLEX NGS Library Prep
NEXTFLEX 16S V1-V3 rRNA Amplicon-seq Kits, 12 Barcodes
NEXTFLEX NGS Library Prep
Revvity kit box for reagents and consumables
NEXTFLEX NGS Library Prep

Library preparation kits for 16S V1-V3 rRNA bacterial metagenomics analysis for Illumina® and Element Biosciences® NGS platforms.

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Feature Specification
Automation Compatible Yes
Product Group 16S amplicon-seq
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Library preparation kits for 16S V1-V3 rRNA bacterial metagenomics analysis for Illumina® and Element Biosciences® NGS platforms.

View product information
Product variants
Unit Size: 24 rxns
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Barcodes
1 - 12
Part #:
NOVA-4202-02S
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USD 806.82
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Unit Size: 24 rxns
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NOVA-4202-02
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USD 562.02
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Unit Size: 96 rxns
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USD 3,102.84
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Unit Size: 96 rxns
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Unit Size: 192 rxns
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Unit Size: 192 rxns
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Unit Size: 192 rxns
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Unit Size: 192 rxns
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Unit Size: 192 rxns
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NOVA-4202-06S
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Unit Size: 192 rxns
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NOVA-4202-07S
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Unit Size: 192 rxns
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For research use only. Not for use in diagnostic procedures.
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USD 562.02
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NEXTFLEX NGS Library Prep
NEXTFLEX 16S V1-V3 rRNA Amplicon-seq Kits, 12 Barcodes
NEXTFLEX NGS Library Prep
Revvity kit box for reagents and consumables
NEXTFLEX 16S V1-V3 rRNA Amplicon-seq Kits, 12 Barcodes
NEXTFLEX NGS Library Prep
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Product information

  • Overview
  • Specifications
  • Citations

Overview

The NEXTFLEX® 16S V1 - V3 rRNA Amplicon-Seq Library Prep Kit is designed for the preparation of multiplexed amplicon libraries that span the hypervariable domains one through three (V1-V3) of microbial 16S ribosomal RNA (rRNA) genes. These libraries are compatible with paired-end sequencing on the Illumina® sequencing platforms.

  • Up to 384 barcodes for multiplexing
  • Practical kit sizes for low and high‑throughput projects
  • Streamlined workflow
  • Automated on Revvity’s suite of liquid handlers to increase your throughput

By utilizing NGS, entire bacterial communities within a sample can be efficiently analyzed from minimal quantities of DNA, without biases that could potentially be introduced by culturing in other non-NGS methods. The NEXTFLEX 16S rRNA amplicon-seq kits maximize the economy and speed of targeted sequencing by offering exceptional values for labs needing a metagenomics solution.

Analysis powered by Cosmos-Hub®

The NEXTFLEX 16S V1 - V3 rRNA Amplicon-seq panels are now available bundled with access to Cosmos-Hub; an online software solution that enables fast and easy analysis of complex microbiome data. Cosmos-Hub gives scientists user-friendly access to version-controlled and validated 16S pipelines. The machine-learning powered software also enables rapid data interpretation through a comparative analysis software with features such as tables, heatmaps, bar charts, multiple Alpha & Beta Diversity indexes, abundance distribution plots, differential abundance testing, as well as comprehensive statistics between groups. Learn more about Cosmos-Hub.

Revvity 16S rRNA analysis - online demonstration

Now you can schedule a one-on-one 30-minute online demonstration that will cover everything you need to know about the Cosmos-Hub® which is now available with Revvity’s NEXTFLEX™ 16S rRNA Amplicon-seq Kits. You will be able to see the analysis demonstrated and our experts will be able to answer any questions that you may have. Please choose a timeframe that suits you best and we will provide you a Microsoft Teams personal link to join when the time comes. We look forward to speaking with you.

Specifications

Analysis
Not Included
Automation Compatible
Yes
Barcodes
1 - 12
Product Group
16S amplicon-seq
Shipping Conditions
Shipped in Dry Ice
Unit Size
24 rxns

Citations

  • L’Heureux, J. E., Corbett, A., Ballard, C., … & Vanhatalo, A. (2025). Oral microbiome and nitric oxide biomarkers in older individuals with APOE4 genotype and mild cognitive impairment. PNAS Nexus, 4(1), pgae543. https://doi.org/10.1093/pnasnexus/pgae543
  • Vanhatalo, A., Curtis, K. J., Littlewood, J., … & James, P. E. (2021). Network analysis of the nitrate-sensitive oral microbiome reveals interactions with cognitive and cardiovascular health. Free Radical Biology and Medicine, 168, 38–52. https://doi.org/10.1016/j.freeradbiomed.2021.02.010
  • Qu, W., Zhou, Y., & Zhang, Y. (2024). Gut microbiota carcinogen metabolism causes distal tissue tumours. Nature, 616, 321–329. https://doi.org/10.1038/s41586-024-07754-w

Resources

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Infographic
Limitations in metagenomics analysis: a roadblock to discoveries

Metagenomics holds tremendous promise, yet it is not without its constraints, especially when it comes to the scarcity of powerful...

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