Signal transducer and activator of transcription 6 (STAT6) is a transcription factor that is a member of the STAT family of proteins. In response to cytokines and growth factors binding to their receptors, STAT6 is phosphorylated by JAK kinases. Once phosphorylated, STAT6 proteins form homo or heterodimers and translocate into the nucleus, where they mediate cytokine induced gene expression. It has been demonstrated that TBK1-induced STAT6 phosphorylation is connected to the STING pathway, contributing to innate immunity's reaction to viral infection. Overexpression of STAT6 plays a direct role in tumorigenesis in leukemia, lymphoma, and prostate and colon cancers.

The Multiplex SureFire Ultra Human Phospho-STAT6 (Tyr641)/Total STAT6 Detection Kit kit is used to measure both phosphorylated and total levels of endogenous STAT6 in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated DAP12 analysis, and the 545 nm (Tb) signal corresponds to the total STAT6 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

• No-wash steps, no separation steps
• Multiplexing assay
• Sensitive detection
• No interference from exogenous antibodies
• Results in less than 3 hours

In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.