Lymphocyte cytosolic protein 2 (SLP-76 or LCP2) is a signal-transducing adaptor protein. ZAP-70 phosphorylates SLP-76, activating SLP-76 which then translocates to the plasma membrane and promotes a multi-protein complex, which participates in the activation, survival, and proliferation of T-Lymphocytes. SLP-76 is involved in multiple sclerosis, rheumatoid arthritis, psoriasis, and many types of cancers.

The AlphaLISA SureFire Ultra Multiplex Human and Mouse Phospho-SLP-76 (Ser376)/Total SLP-76 Detection Kit is used to measure both phosphorylated and total levels of endogenous SLP-76 in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated SLP-76 analysis, and the 545 nm (Tb) signal corresponds to the total SLP-76 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

• No-wash steps, no separation steps
• Multiplexing assay
• Sensitive detection
• No interference from exogenous antibodies
• Results in less than 3 hours

In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.