Positive control lysate for AlphaLISA® SureFire® Ultra™ Multiplex Phospho-Rb (Thr821/826)/Total Rb Detection Kit.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
The positive control lysate is prepared from Jurkat cells cultured in T175 flasks to approximately 1X106 cells/mL in 10% FBS containing medium, then treated with 1.25 mM Pervanadate for 30 minutes and lysed at 3.2 x 106 cells/mL with 1X SureFire Ultra Lysis buffer.
Application |
Protein Detection
|
---|---|
Assay Technology |
Alpha
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra Multiplex
|
Detection Method |
Alpha
|
Experimental Type |
In vitro
|
Unit Size |
1 vial
|
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