Skip to main content
Menu
US

AlphaLISA SureFire Ultra Multiplex Human Phospho-Rb (Ser807/811 & Total) Detection Kit, 10,000 Assay Points

The AlphaLISA™ SureFire® Ultra™ Multiplex phospho-Rb (Ser807/811) + Total Rb assay kit is used to measure both the phosphorylation of Rb and total levels of endogenous Rb in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Rb phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Feature Specification
Application Cell Signaling
Sample Volume 10 µL

The AlphaLISA™ SureFire® Ultra™ Multiplex phospho-Rb (Ser807/811) + Total Rb assay kit is used to measure both the phosphorylation of Rb and total levels of endogenous Rb in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Rb phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 500 Assay Points
Part #:
MPSU-PTRB-M500
List Price
USD 4,015.00
Unit Size: 10,000 Assay Points
Part #:
MPSU-PTRB-M10K
List Price
USD 24,340.00
Unit Size: 50,000 Assay Points
Part #:
MPSU-PTRB-M50K
List Price
USD 76,160.00
Unit Size: 100 Assay Points
Part #:
MPSU-PTRB-M-HV
List Price
USD 1,188.00

Overview

The Alpha SureFire® Ultra™ Multiplex p-Rb (Ser807/811) (Eu) + Total Rb ssay kit is used to measure both phosphorylated and total levels of endogenous Rb in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated Rb analysis, and the 545 nm (Tb) signal corresponds to the total Rb analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

  • No-wash steps, no separation steps
  • Multiplexing assay
  • Sensitive detection
  • No interference from exogenous antibodies
  • Results in less than 3 hours

In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.

Specifications

Application
Cell Signaling
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra Multiplex
Detection Modality
Alpha
Lysis Buffer Compatibility
Lysis Buffer
Molecular Modification
Phosphorylation
Product Group
Kit
Sample Volume
10 µL
Shipping Conditions
Shipped in Blue Ice
Target
RB
Target Class
Phosphoproteins
Target Species
Human
Technology
Alpha
Therapeutic Area
Oncology
Unit Size
10,000 Assay Points

Image gallery

Video gallery

Resources

Are you looking for resources, click on the resource type to explore further.

1-4 of 4 Resources
Guide Icon
Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

Guide Icon
Guide
AlphaPlex quick start guide

The AlphaPlex™ reagent system was designed to enable fast and easy transition from well-established AlphaLISA™ assays to...

Brochure Icon
Brochure
Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...

Flyer Icon
Flyer
Measure more analytes in each well.

Now you can quantify multiple analytes in a single well for faster and more accurate results, even with low sample volumes

Scroll Icon