Positive control lysate for AlphaLISA™ SureFire® Ultra™ Multiplex Phospho-Rb (Ser780)/Total Rb Detection Kit.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Cell Signaling |
Positive control lysate for AlphaLISA™ SureFire® Ultra™ Multiplex Phospho-Rb (Ser780)/Total Rb Detection Kit.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
The positive control lysate is prepared from Jurkat cells cultured in T175 flasks to approximately 1 X 106 cells/mL in 10% FBS containing medium, then treated with 1.25 mM Pervanadate for 30 minutes and lysed at 3.2 x 106 cells/mL with 1X SureFire Ultra Lysis buffer.
Application |
Cell Signaling
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra Multiplex
|
Detection Modality |
Alpha
|
Lysis Buffer Compatibility |
Lysis Buffer
|
Molecular Modification |
Phosphorylation
|
Product Group |
Control Lysate
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
Rb
|
Target Class |
Phosphoproteins
|
Target Species |
Human
|
Technology |
Alpha
|
Unit Size |
1 Vial
|
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