Positive control lysate for AlphaLISA™ SureFire® Ultra™ Human Phospho-DAP12 (Tyr91)/Total DAP12 Detection Kit
For research use only. Not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Cell Signaling |
Positive control lysate for AlphaLISA™ SureFire® Ultra™ Human Phospho-DAP12 (Tyr91)/Total DAP12 Detection Kit
For research use only. Not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
The control lysate is prepared from THP 1 cells cultured in 10% FBS containing media. Cells were harvested and washed with HBSS + 0.1% BSA, adjusted to 4 x10⁶ cells/mL and then treated with 0.5 mM Pervanadate for 30 minutes. Cells were then lysed with the addition of 5X Lysis Buffer.
Application |
Cell Signaling
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra Multiplex
|
Detection Modality |
Alpha
|
Lysis Buffer Compatibility |
Lysis Buffer
|
Molecular Modification |
Phosphorylation
|
Product Group |
Control Lysate
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
DAP12
|
Target Class |
Phosphoproteins
|
Technology |
Alpha
|
Therapeutic Area |
Neuroscience
|
Unit Size |
1 Vial
|
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