AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-c-myc-conjugated AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
This bead kit can be used for many applications, including:
In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The other biomolecule is c-myc-tagged, and associates with the AlphaScreen anti-c-myc Acceptor beads. If the two biomolecules bind to each other, the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction. Competition formats are also possible, using the biotinylated c-myc probe provided in the kit.
Antibody Conjugates |
Streptavidin/Anti-c-Myc
|
---|---|
Application |
Protein-protein interactions
|
Assay Points |
50000
|
Automation Compatible |
Yes
|
Brand |
AlphaScreen
|
One Unit Contains |
50000.0 assay points
|
Species |
Human
|
Shipping Conditions |
Shipped in Blue Ice
|
Unit Size |
50,000 Assay Points
|
Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA...
Are you looking for technical documents related to the product? We have categorized them in dedicated sections below. Explore now.
We are here to answer your questions.